• Chromatin immunoprecipitation analysis of extracts of K-562 cells, using TFAM antibody (STJ115513) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
  • Immunofluorescence analysis of PC-12 cells using TFAM Rabbit polyclonal antibody (STJ115513) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using TFAM Rabbit polyclonal antibody (STJ115513) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of TFAM in paraffin-embedded human colon using TFAM Rabbit polyclonal antibody (STJ115513) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TFAM in paraffin-embedded rat brain using TFAM Rabbit polyclonal antibody (STJ115513) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using TFAM Rabbit polyclonal antibody (STJ115513) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-TFAM antibody (100-200) (STJ115513)

SKU:
STJ115513

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TFAM (100-200) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TFAM
Gene ID: 7019
Uniprot ID: TFAM_HUMAN
Immunogen Region: 100-200
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 100-200 of human TFAM (NP_003192.1).
Immunogen Sequence: QDAYRAEWQVYKEEISRFKE QLTPSQIMSLEKEIMDKHLK RKAMTKKKELTLLGKPKRPR SAYNVYVAERFQEAKGDSPQ EKLKTVKENWKNLSDSEKEL Y
Post Translational Modifications Phosphorylation by PKA within the HMG box 1 impairs DNA binding and promotes degradation by the AAA+ Lon protease.
Function Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA. In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Protein Name Transcription Factor A - Mitochondrial
Mttfa
Mitochondrial Transcription Factor 1
Mttf1
Transcription Factor 6
Tcf-6
Transcription Factor 6-Like 2
Database Links Reactome: R-HSA-163282
Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Transcription Factor A - Mitochondrial antibody
Anti-Mttfa antibody
Anti-Mitochondrial Transcription Factor 1 antibody
Anti-Mttf1 antibody
Anti-Transcription Factor 6 antibody
Anti-Tcf-6 antibody
Anti-Transcription Factor 6-Like 2 antibody
Anti-TFAM antibody
Anti-TCF6 antibody
Anti-TCF6L2 antibody

Information sourced from Uniprot.org

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