• Immunohistochemistry analysis of TEX101 in paraffin-embedded Mouse testis using TEX101 Rabbit polyclonal antibody (STJ11100089) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TEX101 in paraffin-embedded Rat testis using TEX101 Rabbit polyclonal antibody (STJ11100089) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of TEX101 in paraffin-embedded Human colon using TEX101 Rabbit polyclonal antibody (STJ11100089) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from A-549 cells, using TEX101 Rabbit polyclonal antibody (STJ11100089) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-TEX101 antibody (44-150) (STJ11100089)

SKU:
STJ11100089

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TEX101 (44-150) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TEX101
Gene ID: 83639
Uniprot ID: TX101_HUMAN
Immunogen Region: 44-150
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 44-150 of human TEX101 (NP_113639.4).
Immunogen Sequence: LYCQKGLSMTVEADPANMFN WTTEEVETCDKGALCQETIL IIKAGTETAILATKGCIPEG EEAITIVQHSSPPGLIVTSY SNYCEDSFCNDKDSLSQFWE FSETTAS
Tissue Specificity Detected in testis and spermatogonia. Not detected in spermatocytes. Detected in blood leukocytes.
Post Translational Modifications N-glycosylated.by high mannose and/or biantennary complex and/or certain types of hybrid oligosaccharides.possesses different oligosaccharides chains according to its subcellular localization in the testis. Sheds from membrane raft by ACE and released from the cell surface of epididymal sperm while it passes through the caput epididymis leading to disappearance of TEX101 on spermatozoa.is essential to produce fertile spermatozoa.
Function Plays a role in fertilization by controlling binding of sperm to zona pellucida and migration of spermatozoa into the oviduct. May play a role in signal transduction and promote protein tyrosine phosphorylation.
Protein Name Testis-Expressed Protein 101
Cell Surface Receptor Nyd-Sp8
Scleroderma-Associated Autoantigen
Spermatogenesis-Related Gene Protein
Database Links Reactome: R-HSA-163125
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Membrane Raft
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Secreted
Located On Plasma Membrane Of Spermatocytes
Round And Elongated Spermatids
And Testicular Spermatozoa
Alternative Antibody Names Anti-Testis-Expressed Protein 101 antibody
Anti-Cell Surface Receptor Nyd-Sp8 antibody
Anti-Scleroderma-Associated Autoantigen antibody
Anti-Spermatogenesis-Related Gene Protein antibody
Anti-TEX101 antibody
Anti-SGRG antibody
Anti-UNQ867 antibody
Anti-PRO1884 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance