Anti-TET2 antibody (1833-2002) (STJ27649)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TET2 (1833-2002) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TET2 |
| Gene ID | 54790 |
| Uniprot ID | TET2_HUMAN |
| Immunogen Region | 1833-2002 |
| Immunogen Sequence | VQGVASGAEDNDEVWSDSEQ SFLDPDIGGVAVAPTHGSIL IECAKRELHATTPLKNPNRN HPTRISLVFYQHKSMNEPKH GLALWEAKMAEKAREKEEEC EKYGPDYVPQKSHGKKVKRE PAEPHETSEPTYLRFIKSLA ERTMSVTTDSTVTTSPYAFT RVTGPYNRYI |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1833-2002 of human TET2 (NP_001120680.1). |
Additional Info
| Tissue Specificity | Broadly expressed. Highly expressed in hematopoietic cells.highest expression observed in granulocytes. Expression is reduced in granulocytes from peripheral blood of patients affected by myelodysplastic syndromes. |
| Post Translational Modifications | May be glycosylated. It is unclear whether interaction with OGT leads to GlcNAcylation. According to a report, it is not GlcNAcylated by OGT. In contrast, another group reports GlcNAcylation by OGT in mouse ortholog. Monoubiquitinated at Lys-1299 by the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex called CRL4(VprBP) or CUL4A-RBX1-DDB1-DCAF1/VPRBP complex.this modification promotes binding to DNA. Acetylated. Deacetylase HDAC6 acts as a valine sensor by binding to valine through its primate-specific SE14 repeat region and deacetylates TET2 following valine deprivation which promotes TET2-dependent DNA demethylation. |
| Function | Dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. Has a preference for 5-hydroxymethylcytosine in CpG motifs. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. |
| Protein Name | Methylcytosine Dioxygenase Tet2 |
| Database Links | Reactome: R-HSA-5221030Reactome: R-HSA-9827857 |
| Cellular Localisation | NucleusChromosomeLocalization To Chromatin Depends Upon Monoubiquitination At Lys-1299 |
| Alternative Antibody Names | Anti-Methylcytosine Dioxygenase Tet2 antibodyAnti-TET2 antibodyAnti-KIAA1546 antibodyAnti-Nbla00191 antibody |
Information sourced from Uniprot.org