Immunofluorescence – anti-tdTomato antibody using hCEC cells transduced with tdTomato-CAV1; cells were fixed with methanol and anti-tdTomato at 1/250

Anti-tdTomato antibody (STJ140278)

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STJ140278
£94.50 - £440.50
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Host: Goat
Applications: WB/IF/IHC-P/IHC-F/IEM
Reactivity: tdTomato/mCherry/RFP
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description : Goat polyclonal anti-tdTomato for use in WB, IF, IHC-P, IHC-F and IEM in tdTomato, mCherry and RFP samples. Datasheet included with dilution recommendations, and related reagents.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: NaHCO3 100 mM pH 9.
Purification: Epitope affinity purified
Concentration: 2 mg/mL
Dilution Range: WB 1:500-1:5000
IHC-F 1:50-1:500
IHC-P 1:50-1:500
IF 1:50-1:500
IEM 1:50-1:500
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 54 kDa by Western blot. It also detects tdTomato in brain sections by IHC. This antibody is specific for tdTomato and mCherry proteins. It does not cross-react to GFP (green fluorescent p
Immunogen: Purified recombinant peptide produced in E. coli.
Immunogen Sequence: MVSKGEEDNMAIIKEFMRFK VSKGEEDNMAIIKEFMRFKV SKGEEDNMAIIKEFMRFKVH KGEEDNMAIIKEFMRFKVHM GEEDNMAIIKEFMRFKVHME EEDNMAIIKEFMRFKVHMEG EDNMAIIKEFMRFKVHMEGS DNMAIIKEFMRFKVHMEGSV NMAIIKEFMRFKVHMEGSVN MAIIKEFMRFKVHMEGSVNG AIIKEFMRFKVHMEGSVNGH IIKEFMRFKVHMEGSVNGH

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