Anti-TDP1 antibody (1-140) (STJ116265)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TDP1 (1-140) antibody for use in WB, IF, ICC and ELISA in human and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TDP1 |
| Gene ID | 55775 |
| Uniprot ID | TYDP1_HUMAN |
| Immunogen Region | 1-140 |
| Immunogen Sequence | MSQEGDYGRWTISSSDESEE EKPKPDKPSTSSLLCARQGA ANEPRYTCSEAQKAAHKRKI SPVKFSNTDSVLPPKRQKSG SQEDLGWCLSSSDDELQPEM PQKQAEKVVIKKEKDISAPN DGTAQRTENHGAPACHRLKE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human TDP1 (NP_060789.2). |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. |
| Post Translational Modifications | Phosphorylated on serine and/or threonine residues, but not on tyrosine residues. |
| Function | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. |
| Protein Name | Tyrosyl-Dna Phosphodiesterase 1Tyr-Dna Phosphodiesterase 1 |
| Database Links | Reactome: R-HSA-5693571 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Antibody Names | Anti-Tyrosyl-Dna Phosphodiesterase 1 antibodyAnti-Tyr-Dna Phosphodiesterase 1 antibodyAnti-TDP1 antibody |
Information sourced from Uniprot.org