Western blot analysis of various lysates using TCF7L1 Rabbit pAb (STJ11105742) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-TCF7L1 antibody (260-340) (STJ11105742)

⇓ Datasheet ⇓ SDS
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
STJ11105742
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General Information

Short DescriptionRabbit polyclonal Transcription Factor 7-Like 1 (260-340) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB/ELISA
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityPolyclonal
IsotypeIgG
ConjugationUnconjugated
ConcentrationLot specific
PurificationAffinity purification
Dilution RangeWB:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
FormulationPBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Storage InstructionStore at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTCF7L1
Gene ID83439
Uniprot IDTF7L1_HUMAN
Immunogen Region260-340
Immunogen SequenceYSLPPGGFRHPYPALAMNAS MSSLVSSRFSPHMVAPAHPG LPTSGIPHPAIVSPIVKQEP APPSLSPAVSVKSPVTVKKE E
SpecificityRecombinant fusion protein containing a sequence corresponding to amino acids 260-340 of human TCF7L1 (NP_112573.1).

Additional Info

Tissue Specificity Detected in hair follicles and skin keratinocytes, and at lower levels in stomach epithelium.
Function Participates in the Wnt signaling pathway. Binds to DNA and acts as a repressor in the absence of CTNNB1, and as an activator in its presence. Necessary for the terminal differentiation of epidermal cells, the formation of keratohyalin granules and the development of the barrier function of the epidermis. Down-regulates NQO1, leading to increased mitomycin c resistance.
Protein Name Transcription Factor 7-Like 1
Hmg Box Transcription Factor 3
Tcf-3
Database Links Reactome: R-HSA-201722
Reactome: R-HSA-3769402
Reactome: R-HSA-4086398
Reactome: R-HSA-4411364
Reactome: R-HSA-4641265
Reactome: R-HSA-8951430
Reactome: R-HSA-9825892
Reactome: R-HSA-9834899
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor 7-Like 1 antibody
Anti-Hmg Box Transcription Factor 3 antibody
Anti-Tcf-3 antibody
Anti-TCF7L1 antibody
Anti-TCF3 antibody

Information sourced from Uniprot.org

Citations

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