• Immunofluorescence analysis of U-2 OS cells using TCF4 Rabbit polyclonal antibody (STJ25793) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH-3T3 cells using TCF4 Rabbit polyclonal antibody (STJ25793) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using TCF4 Rabbit polyclonal antibody (STJ25793) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from 293T cells, using TCF4 Rabbit polyclonal antibody (STJ25793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TCF4 antibody (1-210) (STJ25793)

SKU:
STJ25793

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TCF4 (1-210) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TCF4
Gene ID: 6925
Uniprot ID: ITF2_HUMAN
Immunogen Region: 1-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human TCF4 (NP_003190.1).
Immunogen Sequence: MHHQQRMAALGTDKELSDLL DFSAMFSPPVSSGKNGPTSL ASGHFTGSNVEDRSSSGSWG NGGHPSPSRNYGDGTPYDHM TSRDLGSHDNLSPPFVNSRI QSKTERGSYSSYGRESNLQG CHQQSLLGGDMDMGNPGTLS PTKPGSQYYQYSSNNPRRRP LHSSAMEVQTKKVRKVPPGL PSSVYAPSASTADYNRDSPG YPSSKPATST
Tissue Specificity Expressed in adult heart, brain, placenta, skeletal muscle and to a lesser extent in the lung. In developing embryonic tissues, expression mostly occurs in the brain.
Function Transcription factor that binds to the immunoglobulin enhancer Mu-E5/KE5-motif. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3'). Binds to the E-box present in the somatostatin receptor 2 initiator element (SSTR2-INR) to activate transcription. Preferentially binds to either 5'-ACANNTGT-3' or 5'-CCANNTGG-3'.
Protein Name Transcription Factor 4
Tcf-4
Class B Basic Helix-Loop-Helix Protein 19
Bhlhb19
Immunoglobulin Transcription Factor 2
Itf-2
Sl3-3 Enhancer Factor 2
Sef-2
Database Links Reactome: R-HSA-525793
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Factor 4 antibody
Anti-Tcf-4 antibody
Anti-Class B Basic Helix-Loop-Helix Protein 19 antibody
Anti-Bhlhb19 antibody
Anti-Immunoglobulin Transcription Factor 2 antibody
Anti-Itf-2 antibody
Anti-Sl3-3 Enhancer Factor 2 antibody
Anti-Sef-2 antibody
Anti-TCF4 antibody
Anti-BHLHB19 antibody
Anti-ITF2 antibody
Anti-SEF2 antibody

Information sourced from Uniprot.org

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