• Western blot analysis of extracts of various cell lines, using TCEB1 antibody (STJ114389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-TCEB1 antibody (1-112) (STJ114389)

SKU:
STJ114389

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TCEB1 (1-112) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ELOC
Gene ID: 6921
Uniprot ID: ELOC_HUMAN
Immunogen Region: 1-112
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-112 of human TCEB1 (NP_001191791.1).
Immunogen Sequence: MDGEEKTYGGCEGPDAMYVK LISSDGHEFIVKREHALTSG TIKAMLSGPGQFAENETNEV NFREIPSHVLSKVCMYFTYK VRYTNSSTEIPEFPIAPEIA LELLMAANFLDC
Tissue Specificity Overexpressed in prostate cancer cell line PC-3 and breast cancer cell line SK-BR-3.
Post Translational Modifications Ubiquitinated by the DCX(AMBRA1) complex, leading to its degradation by the proteasome.
Function SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex). In embryonic stem cells, the elongin BC complex is recruited by EPOP to Polycomb group (PcG) target genes in order generate genomic region that display both active and repressive chromatin properties, an important feature of pluripotent stem cells. Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. This includes the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes. As part of a multisubunit ubiquitin ligase complex composed of elongin BC complex (ELOB and ELOC), elongin A/ELOA, RBX1 and CUL5.polyubiquitinates monoubiquitinated POLR2A. A number of ECS complexes (containing either KLHDC2, KLHDC3, KLHDC10, APPBP2, FEM1A, FEM1B or FEM1C as substrate-recognition component) are part of the DesCEND (destruction via C-end degrons) pathway, which recognizes a C-degron located at the extreme C terminus of target proteins, leading to their ubiquitination and degradation. ECS(LRR1) ubiquitinates MCM7 and promotes CMG replisome disassembly by VCP and chromatin extraction during S-phase.
Protein Name Elongin-C
Eloc
Elongin 15 Kda Subunit
Rna Polymerase Ii Transcription Factor Siii Subunit C
Siii P15
Transcription Elongation Factor B Polypeptide 1
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-1234176
Reactome: R-HSA-167152
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-180585
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-75955
Reactome: R-HSA-8951664
Reactome: R-HSA-9010553
Reactome: R-HSA-9705462
Reactome: R-HSA-983168
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Elongin-C antibody
Anti-Eloc antibody
Anti-Elongin 15 Kda Subunit antibody
Anti-Rna Polymerase Ii Transcription Factor Siii Subunit C antibody
Anti-Siii P15 antibody
Anti-Transcription Elongation Factor B Polypeptide 1 antibody
Anti-ELOC antibody
Anti-TCEB1 antibody

Information sourced from Uniprot.org

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