• Immunohistochemistry analysis of paraffin-embedded rat spleen using TC10/RHOQ Rabbit monoclonal antibody (STJ11103134) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of Rat heart, using TC10/RHOQ Rabbit monoclonal antibody (STJ11103134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of extracts of various cell lines, using TC10/RHOQ Rabbit monoclonal antibody (STJ11103134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TC10/RHOQ antibody (100-205) [S4MR] (STJ11103134)

SKU:
STJ11103134

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-TC10/RHOQ (100-205) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RHOQ
Gene ID: 23433
Uniprot ID: RHOQ_HUMAN
Immunogen Region: 100-205
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 100-205 of human TC10/RHOQ (P17081).
Immunogen Sequence: KEEWVPELKEYAPNVPFLLI GTQIDLRDDPKTLARLNDMK EKPICVEQGQKLAKEIGACC YVECSALTQKGLKTVFDEAI IAILTPKKHTVKKRIGSRCI NCCLIT
Post Translational Modifications May be post-translationally modified by both palmitoylation and polyisoprenylation.
Function Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. May play a role in CFTR trafficking to the plasma membrane. Causes the formation of thin, actin-rich surface projections called filopodia.
Protein Name Rho-Related Gtp-Binding Protein Rhoq
Ras-Like Protein Tc10
Ras-Like Protein Family Member 7a
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-5627083
Reactome: R-HSA-9013406
Cellular Localisation Cytoplasm
Cell Membrane
Lipid-Anchor
Alternative Antibody Names Anti-Rho-Related Gtp-Binding Protein Rhoq antibody
Anti-Ras-Like Protein Tc10 antibody
Anti-Ras-Like Protein Family Member 7a antibody
Anti-RHOQ antibody
Anti-ARHQ antibody
Anti-RASL7A antibody
Anti-TC10 antibody

Information sourced from Uniprot.org

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