• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using Thromboxane synthase Rabbit polyclonal antibody (STJ26192) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of HepG2 cells, using Thromboxane synthase antibody (STJ26192) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
  • Western blot analysis of extracts of various cell lines, using Thromboxane synthase antibody (STJ26192) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-TBXAS1 antibody (355-534) (STJ26192)

SKU:
STJ26192

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TBXAS1 (355-534) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TBXAS1
Gene ID: 6916
Uniprot ID: THAS_HUMAN
Immunogen Region: 355-534
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 355-534 of human Thromboxane synthase (NP_001052.2).
Immunogen Sequence: TYLLATNPDCQEKLLREVDV FKEKHMAPEFCSLEEGLPYL DMVIAETLRMYPPAFRFTRE AAQDCEVLGQRIPAGAVLEM AVGALHHDPEHWPSPETFNP ERFTAEARQQHRPFTYLPFG AGPRSCLGVRLGLLEVKLTL LHVLHKFRFQACPETQVPLQ LESKSALGPKNGVYIKIVSR
Tissue Specificity Platelets, lung, kidney, spleen, macrophages and lung fibroblasts.
Function Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation. Cleaves also PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2. Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE.
Protein Name Thromboxane-A Synthase
Txa Synthase
Txs
Cytochrome P450 5a1
Hydroperoxy Icosatetraenoate Dehydratase
Database Links Reactome: R-HSA-211979
Reactome: R-HSA-2162123
Reactome: R-HSA-5579032
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Alternative Antibody Names Anti-Thromboxane-A Synthase antibody
Anti-Txa Synthase antibody
Anti-Txs antibody
Anti-Cytochrome P450 5a1 antibody
Anti-Hydroperoxy Icosatetraenoate Dehydratase antibody
Anti-TBXAS1 antibody
Anti-CYP5 antibody
Anti-CYP5A1 antibody
Anti-TXAS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance