Anti-Tau antibody (STJ13100493)
SPECIFICATIONS
ClonalityPolyclonal
HostGuinea Pig
ConjugationUnconjugated
ImmunogenA synthetic peptide human Tau conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
General Information
| Short Description | Guinea Pig polyclonal anti-Tau for use in IHC and WB in Human, Rat, Mouse and Marmoset samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Guinea Pig |
| Reactivity | Human/Rat/Mouse/Marmoset |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Conjugation | Unconjugated |
| Purification | Affinity purification |
| Dilution Range | IHC, WB. A dilution of 1:500 is recommended for WB and IHC-P. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | MaptUniprot ID=TAU_RAT" |
| Immunogen | A synthetic peptide human Tau conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse. |
| Specificity | Specific for Tau. |
Additional Info
| Post Translational Modifications | Polyubiquitinated. Requires functional TRAF6 and may provoke SQSTM1-dependent degradation by the proteasome. Phosphorylated at various serine and threonine residues in S-P or T-P motifs by proline-directed protein kinases (PDPK1, CDK1, CDK5, GSK3, MAPK) (a few sites per protein in interphase, more in mitosis), and at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1, MARK2, MARK3, MARK4), causing detachment from microtubules, and their disassembly. Fetal Tau is much more phosphorylated than adult Tau. Phosphorylation at Ser-573 by BRSK1 and BRSK2 in neurons affects ability to bind microtubules and plays a role in neuron polarization. Phosphorylated by PHK. Dephosphorylation at several serine and threonine residues by the serine/threonine phosphatase PPP5C. Phosphorylation at Ser-204 by SGK1 mediates microtubule depolymerization and neurite formation in hippocampal neurons. |
| Function | Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. |
| Protein Name | Microtubule-Associated Protein TauNeurofibrillary Tangle ProteinPaired Helical Filament-TauPhf-Tau |
| Cellular Localisation | CytoplasmCytosolCell MembranePeripheral Membrane ProteinCytoplasmic SideCytoskeletonCell ProjectionAxonDendriteSecretedMostly Found In The Axons Of NeuronsIn The Cytosol And In Association With Plasma Membrane ComponentsCan Be SecretedThe Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein Tau antibodyAnti-Neurofibrillary Tangle Protein antibodyAnti-Paired Helical Filament-Tau antibodyAnti-Phf-Tau antibodyAnti-Mapt antibodyAnti-Mtapt antibodyAnti-Tau antibody |
Information sourced from Uniprot.org