• Immunohistochemistry analysis of paraffin-embedded mouse kidney using TAPBP Antibody (STJ26188) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human gastric cancer using TAPBP Antibody (STJ26188) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat testis using TAPBP Antibody (STJ26188) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of HepG2, using TAPBP Rabbit polyclonal antibody (STJ26188) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-TAPBP antibody (30-260) (STJ26188)

SKU:
STJ26188

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAPBP (30-260) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:100
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAPBP
Gene ID: 6892
Uniprot ID: TPSN_HUMAN
Immunogen Region: 30-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-260 of human TAPBP (NP_757345.2).
Immunogen Sequence: VEDASGKGLAKRPGALLLRQ GPGEPPPRPDLDPELYLSVH DPAGALQAAFRRYPRGAPAP HCEMSRFVPLPASAKWASGL TPAQNCPRALDGAWLMVSIS SPVLSLSSLLRPQPEPQQEP VLITMATVVLTVLTHTPAPR VRLGQDALLDLSFAYMPPTS EAASSLAPGPPPFGLEWRRQ HLGKGHLLLAATPGLNGQMP AAQEGAVAFAAWDDDEPWGP WTGNGTFWLPT
Tissue Specificity Neutrophils, mostly in fully differentiated cells.
Function Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading).
Protein Name Tapasin
Tpn
Tpsn
Ngs-17
Tap-Associated Protein
Tap-Binding Protein
Database Links Reactome: R-HSA-1236974
Reactome: R-HSA-983170
Cellular Localisation Endoplasmic Reticulum Membrane
Single-Pass Type I Membrane Protein
Alternative Antibody Names Anti-Tapasin antibody
Anti-Tpn antibody
Anti-Tpsn antibody
Anti-Ngs-17 antibody
Anti-Tap-Associated Protein antibody
Anti-Tap-Binding Protein antibody
Anti-TAPBP antibody
Anti-NGS17 antibody
Anti-TAPA antibody

Information sourced from Uniprot.org

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