• Western blot analysis of extracts from normal (control) and TAGLN2 knockout (KO) 293T cells, using TAGLN2 antibody (STJ28844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Western blot analysis of extracts of various cell lines, using TAGLN2 antibody (STJ28844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Immunofluorescence analysis of BALB-3T3 cells using [KO Validated] TAGLN2 rabbit polyclonal antibody (STJ28844) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-TAGLN2 antibody (1-199) (STJ28844)

SKU:
STJ28844

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAGLN2 (1-199) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAGLN2
Gene ID: 8407
Uniprot ID: TAGL2_HUMAN
Immunogen Region: 1-199
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human TAGLN2 (NP_003555.1).
Immunogen Sequence: MANRGPAYGLSREVQQKIEK QYDADLEQILIQWITTQCRK DVGRPQPGRENFQNWLKDGT VLCELINALYPEGQAPVKKI QASTMAFKQMEQISQFLQAA ERYGINTTDIFQTVDLWEGK NMACVQRTLMNLGGLAVARD DGLFSGDPNWFPKKSKENPR NFSDNQLQEGKNVIGLQMGT NRGASQAGMTGYGMPRQIL
Tissue Specificity Expressed in epididymis (at protein level).
Protein Name Transgelin-2
Epididymis Tissue Protein Li 7e
Sm22-Alpha Homolog
Database Links Reactome: R-HSA-114608
Alternative Antibody Names Anti-Transgelin-2 antibody
Anti-Epididymis Tissue Protein Li 7e antibody
Anti-Sm22-Alpha Homolog antibody
Anti-TAGLN2 antibody
Anti-KIAA0120 antibody
Anti-CDABP0035 antibody

Information sourced from Uniprot.org

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