• Western blot analysis of extracts of various cell lines, using TAF15 antibody (STJ110763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.
  • Immunoprecipitation analysis of 200 Mu g extracts of SKOV3 cells using 3 Mu g TAF15 antibody (STJ110763). Western blot was performed from the immunoprecipitate using TAF15 antibody (STJ110763) at a dilution of 1:1000.
  • Immunohistochemistry of paraffin-embedded human liver cancer using TAF15 rabbit polyclonal antibody (STJ110763) at dilution of 1:150 (40x lens).
  • Immunohistochemistry analysis of TAF15 in paraffin-embedded human liver cancer using TAF15 Rabbit polyclonal antibody (STJ110763) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunoprecipitation analysis of 200ug extracts of SKOV3 cells using 3ug TAF15 antibody (STJ110763). Western blot was performed from the immunoprecipitate using TAF15 antibody (STJ110763) at a dilition of 1:1000.
  • Western blot analysis of various lysates using TAF15 Rabbit polyclonal antibody (STJ110763) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 5s.

Anti-TAF15 antibody (1-280) (STJ110763)

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STJ110763

$151.88 - $705.38
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TAF15 (1-280) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TAF15
Gene ID: 8148
Uniprot ID: RBP56_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human TAF15 (NP_631961.1).
Immunogen Sequence: MSDSGSYGQSGGEQQSYSTY GNPGSQGYGQASQSYSGYGQ TTDSSYGQNYSGYSSYGQSQ SGYSQSYGGYENQKQSSYSQ QPYNNQGQQQNMESSGSQGG RAPSYDQPDYGQQDSYDQQS GYDQHQGSYDEQSNYDQQHD SYSQNQQSYHSQRENYSHHT QDDRRDVSRYGEDNRGYGGS QGGGRGRGGYDKDGRGPMTG SSGGDRGGFKNFGGHRDYGP RTDADSESDNSDNNTIFVQ
Tissue Specificity Ubiquitous. Observed in all fetal and adult tissues.
Post Translational Modifications Dimethylated by PRMT1 at Arg-206 to asymmetric dimethylarginine. The methylation may favor nuclear localization and positive regulation of TAF15 transcriptional activity. ADP-ribosylated during genotoxic stress.
Function RNA and ssDNA-binding protein that may play specific roles during transcription initiation at distinct promoters. Can enter the preinitiation complex together with the RNA polymerase II (Pol II).
Protein Name Tata-Binding Protein-Associated Factor 2n
68 Kda Tata-Binding Protein-Associated Factor
Taf(Ii68
Tafii68
Rna-Binding Protein 56
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6804756
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Cytoplasm
Shuttles From The Nucleus To The Cytoplasm
Alternative Antibody Names Anti-Tata-Binding Protein-Associated Factor 2n antibody
Anti-68 Kda Tata-Binding Protein-Associated Factor antibody
Anti-Taf(Ii68 antibody
Anti-Tafii68 antibody
Anti-Rna-Binding Protein 56 antibody
Anti-TAF15 antibody
Anti-RBP56 antibody
Anti-TAF2N antibody

Information sourced from Uniprot.org

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