Anti-TAF1 antibody [R09-7P2] (STJA0037433)

SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human TAF1
STJA0037433
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General Information

Short DescriptionRabbit monoclonal anti-TAF1 for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents.
ApplicationsWB
HostRabbit
ReactivityHuman/Mouse/Rat
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

ClonalityMonoclonal
Clone IDR09-7P2
IsotypeIgG
ConjugationUnconjugated
PurificationAffinity Purified
Dilution RangeWB 1:500-1:1000
FormulationRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionStore at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles.

Target Information

Gene SymbolTAF1
Gene ID6872
Uniprot IDTAF1_HUMAN
ImmunogenA synthesized peptide derived from human TAF1

Additional Info

Post Translational Modifications Phosphorylated by casein kinase II in vitro.
Function The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4.
Protein Name Transcription Initiation Factor Tfiid Subunit 1
Cell Cycle Gene 1 Protein
Tbp-Associated Factor 250 Kda
P250
Transcription Initiation Factor Tfiid 250 Kda Subunit
Taf(Ii250
Tafii-250
Tafii250
Database Links Reactome: R-HSA-167161
Reactome: R-HSA-167162
Reactome: R-HSA-167172
Reactome: R-HSA-674695
Reactome: R-HSA-6804756
Reactome: R-HSA-73776
Reactome: R-HSA-73779
Reactome: R-HSA-75953
Reactome: R-HSA-76042
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Initiation Factor Tfiid Subunit 1 antibody
Anti-Cell Cycle Gene 1 Protein antibody
Anti-Tbp-Associated Factor 250 Kda antibody
Anti-P250 antibody
Anti-Transcription Initiation Factor Tfiid 250 Kda Subunit antibody
Anti-Taf(Ii250 antibody
Anti-Tafii-250 antibody
Anti-Tafii250 antibody
Anti-TAF1 antibody
Anti-BA2R antibody
Anti-CCG1 antibody
Anti-CCGS antibody
Anti-TAF2A antibody

Information sourced from Uniprot.org

Citations

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