Anti-TAF1 antibody (990-1250) (STJ118176)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal TAF1 (990-1250) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | TAF1 |
| Gene ID | 6872 |
| Uniprot ID | TAF1_HUMAN |
| Immunogen Region | 990-1250 |
| Immunogen Sequence | GTDADLRRLSLKNAKQLLRK FGVPEEEIKKLSRWEVIDVV RTMSTEQARSGEGPMSKFAR GSRFSVAEHQERYKEECQRI FDLQNKVLSSTEVLSTDTDS SSAEDSDFEEMGKNIENMLQ NKKTSSQLSREREEQERKEL QRMLLAAGSAASGNNHRDDD TASVTSLNSSATGRCLKIYR TFRDEEGKEYVRCETVRKPA VIDAYVRIRTTKDEEFIRKF ALFDEQHREEMRKERRRIQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 990-1250 of human TAF1 (NP_620278.1). |
Additional Info
| Post Translational Modifications | Phosphorylated by casein kinase II in vitro. |
| Function | The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2. Contains novel N- and C-terminal Ser/Thr kinase domains which can autophosphorylate or transphosphorylate other transcription factors. Phosphorylates TP53 on 'Thr-55' which leads to MDM2-mediated degradation of TP53. Phosphorylates GTF2A1 and GTF2F1 on Ser residues. Possesses DNA-binding activity. Essential for progression of the G1 phase of the cell cycle. Exhibits histone acetyltransferase activity towards histones H3 and H4. |
| Protein Name | Transcription Initiation Factor Tfiid Subunit 1Cell Cycle Gene 1 ProteinTbp-Associated Factor 250 KdaP250Transcription Initiation Factor Tfiid 250 Kda SubunitTaf(Ii250Tafii-250Tafii250 |
| Database Links | Reactome: R-HSA-167161Reactome: R-HSA-167162Reactome: R-HSA-167172Reactome: R-HSA-674695Reactome: R-HSA-6804756Reactome: R-HSA-73776Reactome: R-HSA-73779Reactome: R-HSA-75953Reactome: R-HSA-76042 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Transcription Initiation Factor Tfiid Subunit 1 antibodyAnti-Cell Cycle Gene 1 Protein antibodyAnti-Tbp-Associated Factor 250 Kda antibodyAnti-P250 antibodyAnti-Transcription Initiation Factor Tfiid 250 Kda Subunit antibodyAnti-Taf(Ii250 antibodyAnti-Tafii-250 antibodyAnti-Tafii250 antibodyAnti-TAF1 antibodyAnti-BA2R antibodyAnti-CCG1 antibodyAnti-CCGS antibodyAnti-TAF2A antibody |
Information sourced from Uniprot.org