• Immunofluorescence analysis of U2OS cells using SYT4 Rabbit polyclonal antibody (STJ110048) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SYT4 in paraffin-embedded human liver cancer using SYT4 Rabbit polyclonal antibody (STJ110048) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SYT4 in paraffin-embedded human stomach using SYT4 Rabbit polyclonal antibody (STJ110048) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates, using SYT4 Rabbit polyclonal antibody (STJ110048) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-SYT4 antibody (38-190) (STJ110048)

SKU:
STJ110048

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SYT4 (38-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYT4
Gene ID: 6860
Uniprot ID: SYT4_HUMAN
Immunogen Region: 38-190
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 38-190 of human SYT4 (NP_065834.1).
Immunogen Sequence: CQRKSSKSNKTPPYKFVHVL KGVDIYPENLNSKKKFGADD KNEVKNKPAVPKNSLHLDLE KRDLNGNFPKTNLKPGSPSD LENATPKLFLEGEKESVSPE SLKSSTSLTSEEKQEKLGTL FFSLEYNFERKAFVVNIKEA RGLPAMDEQSMTS
Tissue Specificity Expressed in melanocytes. Expressed in brain. Within brain, expression is highest in hippocampus, with substantial levels also detected in amygdala and thalamus.
Post Translational Modifications Phosphorylation at Ser-135 by MAPK8/JNK1 reduces interaction with KIF1A and neuronal dense core vesicles mobility.
Function Synaptotagmin family member which does not bind Ca(2+). Involved in neuronal dense core vesicles (DCVs) mobility through its interaction with KIF1A. Upon increased neuronal activity, phosphorylation by MAPK8/JNK1 destabilizes the interaction with KIF1A and captures DCVs to synapses. Plays a role in dendrite formation by melanocytes.
Protein Name Synaptotagmin-4
Synaptotagmin Iv
Sytiv
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Neuronal Dense Core Vesicle Membrane
Single-Pass Membrane Protein
Alternative Antibody Names Anti-Synaptotagmin-4 antibody
Anti-Synaptotagmin Iv antibody
Anti-Sytiv antibody
Anti-SYT4 antibody
Anti-KIAA1342 antibody

Information sourced from Uniprot.org

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