• Western blot analysis of extracts of various cell lines, using SYT11 antibody (STJ110497) at 1:600 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunofluorescence analysis of mouse brain cells using SYT11 rabbit polyclonal antibody (STJ110497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain cells using SYT11 rabbit polyclonal antibody (STJ110497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of SH-5Y5Y cells using SYT11 rabbit polyclonal antibody (STJ110497) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SYT11 antibody (37-200) (STJ110497)

SKU:
STJ110497

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SYT11 (37-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYT11
Gene ID: 23208
Uniprot ID: SYT11_HUMAN
Immunogen Region: 37-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-200 of human SYT11 (NP_689493.3).
Immunogen Sequence: WSCCHQQAEKKQKNPPYKFI HMLKGISIYPETLSNKKKII KVRRDKDGPGREGGRRNLLV DAAEAGLLSRDKDPRGPSSG SCIDQLPIKMDYGEELRSPI TSLTPGESKTTSPSSPEEDV MLGSLTFSVDYNFPKKALVV TIQEAHGLPVMDDQTQGSDP YIKM
Post Translational Modifications Ubiquitinated, at least by PRKN, and targeted to the proteasome complex for degradation. Ubiquitination is inhibited by ATP13A2.
Function Synaptotagmin family member involved in vesicular and membrane trafficking which does not bind Ca(2+). Inhibits clathrin-mediated and bulk endocytosis, functions to ensure precision in vesicle retrieval. Plays an important role in dopamine transmission by regulating endocytosis and the vesicle-recycling process. Essential component of a neuronal vesicular trafficking pathway that differs from the synaptic vesicle trafficking pathway but is crucial for development and synaptic plasticity. In macrophages and microglia, inhibits the conventional cytokine secretion, of at least IL6 and TNF, and phagocytosis. In astrocytes, regulates lysosome exocytosis, mechanism required for the repair of injured astrocyte cell membrane. Required for the ATP13A2-mediated regulation of the autophagy-lysosome pathway.
Protein Name Synaptotagmin-11
Synaptotagmin Xi
Sytxi
Database Links Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Cellular Localisation Cytoplasmic Vesicle Membrane
Single-Pass Membrane Protein
Perikaryon
Golgi Apparatus
Trans-Golgi Network Membrane
Recycling Endosome Membrane
Lysosome Membrane
Cytoplasmic Vesicle
Phagosome
Cell Projection
Axon
Dendrite
Postsynaptic Density
Clathrin-Coated Vesicle Membrane
Localized In Vesicles That Travels In Axonal And Dendritic Shafts In Both Anterograde And Retrograde Directions
In Macrophages And Microglia
Recruited In Phagosomes At Early Stages Of Phagocytosis
Found In The Core Of The Lewy Bodies In The Brain Of Sporadic Parkinson Disease Patients
Alternative Antibody Names Anti-Synaptotagmin-11 antibody
Anti-Synaptotagmin Xi antibody
Anti-Sytxi antibody
Anti-SYT11 antibody
Anti-KIAA0080 antibody

Information sourced from Uniprot.org

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