• Confocal imaging of paraffin-embedded Rat brain using Synapsin II Rabbit monoclonal antibody (STJ11103035, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of paraffin-embedded Mouse brain using Synapsin II Rabbit monoclonal antibody (STJ11103035, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Western blot analysis of lysates from 293T cells, using Synapsin II Rabbit monoclonal antibody (STJ11103035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Synapsin II antibody (200-300) [S5MR] (STJ11103035)

SKU:
STJ11103035

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-Synapsin II (200-300) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYN2
Gene ID: 6854
Uniprot ID: SYN2_HUMAN
Immunogen Region: 200-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-300 of human Synapsin II (Q92777).
Immunogen Sequence: HLIIGMQYAGLPSINSLESI YNFCDKPWVFAQLVAIYKTL GGEKFPLIEQTYYPNHKEML TLPTFPVVVKIGHAHSGMGK VKVENHYDFQDIASVVALTQ T
Tissue Specificity Central and peripheral nervous systems.
Post Translational Modifications Phosphorylation at Ser-10 dissociates synapsins from synaptic vesicles. Phosphorylation at Ser-425 by MAPK1/ERK2 and/or MAPK3/ERK1 may play a role in noradrenaline secretion by sympathetic neurons.
Function Neuronal phosphoprotein that coats synaptic vesicles, binds to the cytoskeleton, and is believed to function in the regulation of neurotransmitter release. May play a role in noradrenaline secretion by sympathetic neurons.
Protein Name Synapsin-2
Synapsin Ii
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-212676
Cellular Localisation Synapse
Alternative Antibody Names Anti-Synapsin-2 antibody
Anti-Synapsin Ii antibody
Anti-SYN2 antibody

Information sourced from Uniprot.org

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