• Immunofluorescence analysis of rat brain using SYN1 Rabbit polyclonal antibody (STJ11105704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse brain using SYN1 Rabbit polyclonal antibody (STJ11105704) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of SH-SY5Y, using SYN1 Rabbit polyclonal antibody (STJ11105704) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-SYN1 antibody (400-500) (STJ11105704)

SKU:
STJ11105704

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Synapsin-1 (400-500) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SYN1
Gene ID: 6853
Uniprot ID: SYN1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human SYN1 (NP_008881.2).
Immunogen Sequence: DEDKQLIVELVVNKMAQALP RQRQRDASPGRGSHGQTPSP GALPLGRQTSQQPAGPPAQQ RPPPQGGPPQPGPGPQRQGP PLQQRPPPQGQQHLSGLGPP A
Post Translational Modifications Substrate of different protein kinases. Phosphorylated by CaMK2 and MAPK1. Phosphorylation, including phosphorylation at Ser-9, promotes synapsin-1 dissociation from synaptic vesicles, regulates its rate of dispersion, and controls the kinetics of vesicle pool turnover and neurotransmitter release .
Function Neuronal phosphoprotein that coats synaptic vesicles, and binds to the cytoskeleton. Acts as a regulator of synaptic vesicles trafficking, involved in the control of neurotransmitter release at the pre-synaptic terminal. Also involved in the regulation of axon outgrowth and synaptogenesis. The complex formed with NOS1 and CAPON proteins is necessary for specific nitric-oxid functions at a presynaptic level.
Protein Name Synapsin-1
Brain Protein 4.1
Synapsin I
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-212676
Reactome: R-HSA-9662360
Cellular Localisation Synapse
Golgi Apparatus
Presynapse
Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle
Dissociates From Synaptic Vesicles And Redistributes Into The Axon During Action Potential Firing
In A Step That Precedes Fusion Of Vesicles With The Plasma Membrane
Reclusters To Presynapses After The Cessation Of Synaptic Activity
Alternative Antibody Names Anti-Synapsin-1 antibody
Anti-Brain Protein 4.1 antibody
Anti-Synapsin I antibody
Anti-SYN1 antibody

Information sourced from Uniprot.org

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