• Western blot analysis of extracts of HepG2 cells, using SURF4 antibody (STJ119154) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using SURF4 antibody (STJ119154) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat pancreatic islet using SURF4 antibody (STJ119154) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using SURF4 rabbit polyclonal antibody (STJ119154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using SURF4 rabbit polyclonal antibody (STJ119154) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SURF4 antibody (50-150) (STJ119154)

SKU:
STJ119154

£112.50 - £524.50
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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:50-1:200
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SURF4
Gene ID: 6836
Uniprot ID: SURF4_HUMAN
Immunogen Region: 50-150
Specificity: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SURF4 (NP_001267719.1).
Immunogen Sequence: ACFGLFGIIALQTIAYSILW DLKFLMRNLALGGGLLLLLA ESRSEGKSMFAGVPTMRESS PKQYMQLGGRVLLVLMFMTL LHFDASFFSIVQNIVGTALM I
Function Endoplasmic reticulum cargo receptor that mediates the export of lipoproteins by recruiting cargos into COPII vesicles to facilitate their secretion. Acts as a cargo receptor for lipoproteins bearing both APOB and APOA1, thereby regulating lipoprotein delivery and the maintenance of lipid homeostasis. Synergizes with the GTPase SAR1B to mediate transport of circulating lipoproteins. Promotes the secretion of PCSK9. Also mediates the efficient secretion of erythropoietin (EPO). May also play a role in the maintenance of the architecture of the endoplasmic reticulum-Golgi intermediate compartment and of the Golgi.
Protein Name Surfeit Locus Protein 4
Database Links Reactome: R-HSA-6798695
Reactome: R-HSA-6811434
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Golgi Apparatus Membrane
Active At Endoplasmic Reticulum Exit Sites (Eres) Where It Is Incorporated Together With Its Lipoprotein Cargos Into Copii-Coated Vesicles
From The Golgi It Is Recycled Back To The Endoplasmic Reticulum
Alternative Antibody Names Anti-Surfeit Locus Protein 4 antibody
Anti-SURF4 antibody
Anti-SURF-4 antibody

Information sourced from Uniprot.org

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