• Western blot analysis of extracts of various cell lines, using SUPT5H/SPT5 antibody (STJ111606) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunoprecipitation analysis of 300 Mu g extracts of Hela cells using 3 Mu g SUPT5H/SPT5 rabbit polyclonal antibody (STJ111606). Western blot was performed from the immunoprecipitate using SUPT5H/SPT5 rabbit polyclonal antibody (STJ111606) at a dilition of 1:500.

Anti-SUPT5H antibody (125-425) (STJ111606)

SKU:
STJ111606

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUPT5H (125-425) is suitable for use in Western Blot and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:200-1:2000
IP 1:200-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPT5H
Gene ID: 6829
Uniprot ID: SPT5H_HUMAN
Immunogen Region: 125-425
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 125-425 of human SUPT5H/SPT5 (NP_003160.2).
Immunogen Sequence: LQNLWRDQREEELGEYYMKK YAKSSVGETVYGGSDELSDD ITQQQLLPGVKDPNLWTVKC KIGEERATAISLMRKFIAYQ FTDTPLQIKSVVAPEHVKGY IYVEAYKQTHVKQAIEGVGN LRLGYWNQQMVPIKEMTDVL KVVKEVANLKPKSWVRLKRG IYKDDIAQVDYVEPSQNTIS LKMIPRIDYDRIKARMSLKD WFAKRKKFKRPPQRLFDAEK IRSLGGDVASDGDFLIFEG
Tissue Specificity Ubiquitously expressed.
Post Translational Modifications Methylated by PRMT1/HRMT1L2 and PRMT5/SKB1. Methylation negatively regulates interaction with P-TEFb and RNA polymerase II. Phosphorylated by CDK7 and CDK9. Phosphorylation by P-TEFb alleviates transcriptional pausing and can stimulate transcriptional elongation from the HIV-1 LTR. P-TEFb dependent phosphorylation is stimulated by the HIV-1 Tat protein. Phosphorylation may also stimulate interaction with PIN1. Bulk phosphorylation occurs predominantly in mitosis.
Function Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Protein Name Transcription Elongation Factor Spt5
Hspt5
Drb Sensitivity-Inducing Factor 160 Kda Subunit
Dsif P160
Drb Sensitivity-Inducing Factor Large Subunit
Dsif Large Subunit
Tat-Cotransactivator 1 Protein
Tat-Ct1 Protein
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167160
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6807505
Reactome: R-HSA-72086
Reactome: R-HSA-75955
Reactome: R-HSA-77075
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Elongation Factor Spt5 antibody
Anti-Hspt5 antibody
Anti-Drb Sensitivity-Inducing Factor 160 Kda Subunit antibody
Anti-Dsif P160 antibody
Anti-Drb Sensitivity-Inducing Factor Large Subunit antibody
Anti-Dsif Large Subunit antibody
Anti-Tat-Cotransactivator 1 Protein antibody
Anti-Tat-Ct1 Protein antibody
Anti-SUPT5H antibody
Anti-SPT5 antibody
Anti-SPT5H antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance