• Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-SUPT4H1 antibody (40-120 aa) (STJ190203)

SKU:
STJ190203

Current Stock:
Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Transcription elongation factor SPT4 (40-120 aa) is suitable for use in Western Blot and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-2000
ELISA 1:5000-20000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPT4H1
Gene ID: 6827
Uniprot ID: SPT4H_HUMAN
Immunogen Region: 40-120 aa
Specificity: SPT4H Polyclonal Antibody detects endogenous levels of protein.
Immunogen: Synthesized peptide derived from the human protein at the amino acid range 40-120
Function Component of the DRB sensitivity-inducing factor complex (DSIF complex), which regulates mRNA processing and transcription elongation by RNA polymerase II. DSIF positively regulates mRNA capping by stimulating the mRNA guanylyltransferase activity of RNGTT/CAP1A. DSIF also acts cooperatively with the negative elongation factor complex (NELF complex) to enhance transcriptional pausing at sites proximal to the promoter. Transcriptional pausing may facilitate the assembly of an elongation competent RNA polymerase II complex. DSIF and NELF promote pausing by inhibition of the transcription elongation factor TFIIS/S-II. TFIIS/S-II binds to RNA polymerase II at transcription pause sites and stimulates the weak intrinsic nuclease activity of the enzyme. Cleavage of blocked transcripts by RNA polymerase II promotes the resumption of transcription from the new 3' terminus and may allow repeated attempts at transcription through natural pause sites. DSIF can also positively regulate transcriptional elongation and is required for the efficient activation of transcriptional elongation by the HIV-1 nuclear transcriptional activator, Tat. DSIF acts to suppress transcriptional pausing in transcripts derived from the HIV-1 LTR and blocks premature release of HIV-1 transcripts at terminator sequences.
Protein Name Transcription Elongation Factor Spt4
Hspt4
Drb Sensitivity-Inducing Factor 14 Kda Subunit
Dsif P14
Drb Sensitivity-Inducing Factor Small Subunit
Dsif Small Subunit
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-113418
Reactome: R-HSA-167152
Reactome: R-HSA-167158
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167242
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6807505
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcription Elongation Factor Spt4 antibody
Anti-Hspt4 antibody
Anti-Drb Sensitivity-Inducing Factor 14 Kda Subunit antibody
Anti-Dsif P14 antibody
Anti-Drb Sensitivity-Inducing Factor Small Subunit antibody
Anti-Dsif Small Subunit antibody
Anti-SUPT4H1 antibody
Anti-SPT4H antibody
Anti-SUPT4H antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance