• Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from HepG2 and Jurkat cells, using SUPT16H Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using SPT16 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-SUPT16H antibody (941-990 aa) (STJ95753)

SKU:
STJ95753

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-FACT complex subunit SPT16 (941-990 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUPT16H
Gene ID: 11198
Uniprot ID: SP16H_HUMAN
Immunogen Region: 941-990 aa
Specificity: SPT16 Polyclonal Antibody detects endogenous levels of SPT16 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human SUPT16H at the amino acid range 941-990
Post Translational Modifications ADP-ribosylated. ADP-ribosylation by PARP1 is induced by genotoxic stress and correlates with dissociation of FACT from chromatin.
Function Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II).
Protein Name Fact Complex Subunit Spt16
Chromatin-Specific Transcription Elongation Factor 140 Kda Subunit
Fact 140 Kda Subunit
Factp140
Facilitates Chromatin Transcription Complex Subunit Spt16
Hspt16
Database Links Reactome: R-HSA-112382
Reactome: R-HSA-167152
Reactome: R-HSA-167200
Reactome: R-HSA-167238
Reactome: R-HSA-167243
Reactome: R-HSA-167246
Reactome: R-HSA-167287
Reactome: R-HSA-167290
Reactome: R-HSA-674695
Reactome: R-HSA-6796648
Reactome: R-HSA-6804756
Reactome: R-HSA-75955
Cellular Localisation Nucleus
Chromosome
Colocalizes With Rna Polymerase Ii On Chromatin
Recruited To Actively Transcribed Loci
Alternative Antibody Names Anti-Fact Complex Subunit Spt16 antibody
Anti-Chromatin-Specific Transcription Elongation Factor 140 Kda Subunit antibody
Anti-Fact 140 Kda Subunit antibody
Anti-Factp140 antibody
Anti-Facilitates Chromatin Transcription Complex Subunit Spt16 antibody
Anti-Hspt16 antibody
Anti-SUPT16H antibody
Anti-FACT140 antibody
Anti-FACTP140 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance