Anti-Superoxide dismutase 1 antibody (aa: 24-36) (STJA0040878)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid
General Information
| Short Description | Rabbit polyclonal anti-Superoxide dismutase 1 (aa: 24-36) for use in WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:500-1:1000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | SOD1 |
| Gene ID | 6647 |
| Uniprot ID | SODC_HUMAN |
| Immunogen | A synthetic peptide (ESNGPVK VWGSIK) as part of human superoxide dismutase (SOD1) protein (aa: 24-36) conjugated to diphtheria toxoid |
| Immunogen Region | aa: 24-36 |
| Immunogen Sequence | Human |
| Specificity | Specificity was confirmed by Western blot detecting human superoxide dismutase (SOD1). This antiserum is known to react with human superoxide dismutase (SOD1). |
Additional Info
| Post Translational Modifications | Unlike wild-type protein, the pathogenic variants ALS1 Arg-38, Arg-47, Arg-86 and Ala-94 are polyubiquitinated by RNF19A leading to their proteasomal degradation. The pathogenic variants ALS1 Arg-86 and Ala-94 are ubiquitinated by MARCH5 leading to their proteasomal degradation. The ditryptophan cross-link at Trp-33 is responsible for the non-disulfide-linked homodimerization. Such modification might only occur in extreme conditions and additional experimental evidence is required. Palmitoylation helps nuclear targeting and decreases catalytic activity. Succinylation, adjacent to copper catalytic site, probably inhibits activity. Desuccinylation by SIRT5 enhances activity. |
| Function | Destroys radicals which are normally produced within the cells and which are toxic to biological systems. |
| Protein Name | Superoxide Dismutase Cu-ZnSuperoxide Dismutase 1Hsod1 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-3299685Reactome: R-HSA-8950505 |
| Cellular Localisation | CytoplasmNucleusPredominantly CytoplasmicThe Pathogenic Variants Als1 Arg-86 And Ala-94 Gradually Aggregates And Accumulates In Mitochondria |
| Alternative Antibody Names | Anti-Superoxide Dismutase Cu-Zn antibodyAnti-Superoxide Dismutase 1 antibodyAnti-Hsod1 antibodyAnti-SOD1 antibody |
Information sourced from Uniprot.org