• Immunohistochemistry analysis of SUMO3 in paraffin-embedded human prostate using SUMO3 Rabbit polyclonal antibody (STJ25750) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SUMO3 in paraffin-embedded rat brain using SUMO3 Rabbit polyclonal antibody (STJ25750) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of SUMO3 in paraffin-embedded mouse brain using SUMO3 Rabbit polyclonal antibody (STJ25750) (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from MCF-7 cells, using SUMO3 Rabbit polyclonal antibody (STJ25750) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-SUMO3 antibody (51-69) (STJ25750)

SKU:
STJ25750

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMO3 (51-69) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMO3
Gene ID: 6612
Uniprot ID: SUMO3_HUMAN
Immunogen Region: 51-69
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 51-69 of human SUMO3 (NP_008867.2).
Immunogen Sequence: GLSMRQIRFRFDGQPINET
Tissue Specificity Expressed predominantly in liver.
Post Translational Modifications Polymeric chains can be formed through Lys-11 cross-linking. Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
Function Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4. Plays a role in the regulation of sumoylation status of SETX.
Protein Name Small Ubiquitin-Related Modifier 3
Sumo-3
Smt3 Homolog 1
Sumo-2
Ubiquitin-Like Protein Smt3a
Smt3a
Database Links Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Reactome: R-HSA-3065679
Reactome: R-HSA-3108214
Reactome: R-HSA-3232118
Reactome: R-HSA-3899300
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-4615885
Reactome: R-HSA-4755510
Reactome: R-HSA-5696395
Cellular Localisation Cytoplasm
Nucleus
Pml Body
Alternative Antibody Names Anti-Small Ubiquitin-Related Modifier 3 antibody
Anti-Sumo-3 antibody
Anti-Smt3 Homolog 1 antibody
Anti-Sumo-2 antibody
Anti-Ubiquitin-Like Protein Smt3a antibody
Anti-Smt3a antibody
Anti-SUMO3 antibody
Anti-SMT3A antibody
Anti-SMT3H1 antibody

Information sourced from Uniprot.org

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