• Immunofluorescence analysis of MCF7 cells using SUMO1 Rabbit polyclonal antibody (STJ25747) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of A549 cells using SUMO1 Rabbit polyclonal antibody (STJ25747) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using SUMO1 Rabbit polyclonal antibody (STJ25747) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using SUMO1 Rabbit polyclonal antibody (STJ25747) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SUMO1 antibody (1-101) (STJ25747)

SKU:
STJ25747

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUMO1 (1-101) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
IF/ICC 1:50-1:200
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUMO1
Gene ID: 7341
Uniprot ID: SUMO1_HUMAN
Immunogen Region: 1-101
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human SUMO1 (NP_003343.1).
Immunogen Sequence: MSDQEAKPSTEDLGDKKEGE YIKLKVIGQDSSEIHFKVKM TTHLKKLKESYCQRQGVPMN SLRFLFEGQRIADNHTPKEL GMEEEDVIEVYQEQTGGHST V
Post Translational Modifications Cleavage of precursor form by SENP1 or SENP2 is necessary for function. Polymeric SUMO1 chains undergo polyubiquitination by RNF4.
Function Ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment via an isopeptide bond to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Involved for instance in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Covalently attached to the voltage-gated potassium channel KCNB1.this modulates the gating characteristics of KCNB1. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. May also regulate a network of genes involved in palate development. Covalently attached to ZFHX3.
Protein Name Small Ubiquitin-Related Modifier 1
Sumo-1
Gap-Modifying Protein 1
Gmp1
Smt3 Homolog 3
Sentrin
Ubiquitin-Homology Domain Protein Pic1
Ubiquitin-Like Protein Smt3c
Smt3c
Ubiquitin-Like Protein Ubl1
Database Links Reactome: R-HSA-3065676
Reactome: R-HSA-3065678
Reactome: R-HSA-3065679
Reactome: R-HSA-3108214
Reactome: R-HSA-3232118
Reactome: R-HSA-3232142
Reactome: R-HSA-3899300
Reactome: R-HSA-4085377
Reactome: R-HSA-4090294
Reactome: R-HSA-4551638
Reactome: R-HSA-4570464
Reactome: R-HSA-4615885
Reactome: R-HSA-4655427
Reactome: R-HSA-4755510
Reactome: R-HSA-5693565
Reactome: R-HSA-5696395
Reactome: R-HSA-877312
Reactome: R-HSA-8866904
Reactome: R-HSA-9615933
Reactome: R-HSA-9683610
Reactome: R-HSA-9694631
Reactome: R-HSA-9793242
Cellular Localisation Nucleus Membrane
Nucleus Speckle
Cytoplasm
Nucleus
Pml Body
Cell Membrane
Recruited By Bcl11a Into The Nuclear Body
In The Presence Of Zfhx3
Sequesterd To Nuclear Body (Nb)-Like Dots In The Nucleus Some Of Which Overlap Or Closely Associate With Pml Body
Alternative Antibody Names Anti-Small Ubiquitin-Related Modifier 1 antibody
Anti-Sumo-1 antibody
Anti-Gap-Modifying Protein 1 antibody
Anti-Gmp1 antibody
Anti-Smt3 Homolog 3 antibody
Anti-Sentrin antibody
Anti-Ubiquitin-Homology Domain Protein Pic1 antibody
Anti-Ubiquitin-Like Protein Smt3c antibody
Anti-Smt3c antibody
Anti-Ubiquitin-Like Protein Ubl1 antibody
Anti-SUMO1 antibody
Anti-SMT3C antibody
Anti-SMT3H3 antibody
Anti-UBL1 antibody
Anti-OK antibody
Anti-SW-cl.43 antibody

Information sourced from Uniprot.org

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