• Western blot analysis of extracts of various cell lines, using SUCLG2 antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of U-2 OS cells using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human liver cancer using SUCLG2 antibody (STJ116335) at dilution of 1:100 (40x lens).
  • Immunofluorescence analysis of C6 cells using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry of paraffin-embedded human lung cancer using SUCLG2 antibody (STJ116335) at dilution of 1:100 (40x lens).
  • Immunohistochemistry analysis of SUCLG2 in paraffin-embedded human lung cancer using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of C6 cells using SUCLG2 Polyclonal Antibody (STJ116335) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of SUCLG2 in paraffin-embedded human liver cancer using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using SUCLG2 Polyclonal Antibody (STJ116335) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from wild type (WT) and SUCLG2 knockout (KO) 293T (KO) cells, using [KO Validated] SUCLG2 Rabbit polyclonal antibody (STJ116335) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-SUCLG2 antibody (50-300) (STJ116335)

SKU:
STJ116335

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUCLG2 (50-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUCLG2
Gene ID: 8801
Uniprot ID: SUCB2_HUMAN
Immunogen Region: 50-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-300 of human SUCLG2 (NP_003839.2).
Immunogen Sequence: SDNGVRVQRFFVADTANEAL EAAKRLNAKEIVLKAQILAG GRGKGVFNSGLKGGVHLTKD PNVVGQLAKQMIGYNLATKQ TPKEGVKVNKVMVAEALDIS RETYLAILMDRSCNGPVLVG SPQGGVDIEEVAASNPELIF KEQIDIFEGIKDSQAQRMAE NLGFVGPLKSQAADQITKLY NLFLKIDATQVEVNPFGETP EGQVVCFDAKINFDDNAEFR QKDIFAMDDKSENEPIENE
Tissue Specificity Mainly expressed in liver, kidney, heart, spleen and skeletal muscle. Also found in intestine and colon, and in low amounts in lung, brain, prostate, testis and ovary.
Function GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Protein Name Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial
Gtp-Specific Succinyl-Coa Synthetase Subunit Beta
G-Scs
Gtpscs
Succinyl-Coa Synthetase Beta-G Chain
Scs-Betag
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Succinate--Coa Ligase Gdp-Forming Subunit Beta - Mitochondrial antibody
Anti-Gtp-Specific Succinyl-Coa Synthetase Subunit Beta antibody
Anti-G-Scs antibody
Anti-Gtpscs antibody
Anti-Succinyl-Coa Synthetase Beta-G Chain antibody
Anti-Scs-Betag antibody
Anti-SUCLG2 antibody

Information sourced from Uniprot.org

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