• Western blot analysis of extracts of various cell lines, using SUCLA2 antibody (STJ112080) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunofluorescence analysis of L929 using SUCLA2 antibody (STJ112080) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS using SUCLA2 antibody (STJ112080) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SUCLA2 antibody (1-180) (STJ112080)

SKU:
STJ112080

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SUCLA2 (1-180) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SUCLA2
Gene ID: 8803
Uniprot ID: SUCB1_HUMAN
Immunogen Region: 1-180
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1).
Immunogen Sequence: MAASMFYGRLVAVATLRNHR PRTAQRAAAQVLGSSGLFNN HGLQVQQQQQRNLSLHEYMS MELLQEAGVSVPKGYVAKSP DEAYAIAKKLGSKDVVIKAQ VLAGGRGKGTFESGLKGGVK IVFSPEEAKAVSSQMIGKKL FTKQTGEKGRICNQVLVCER KYPRREYYFAITMERSFQGP
Tissue Specificity Widely expressed. Not expressed in liver and lung.
Function ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.
Protein Name Succinate--Coa Ligase Adp-Forming Subunit Beta - Mitochondrial
Atp-Specific Succinyl-Coa Synthetase Subunit Beta
A-Scs
Succinyl-Coa Synthetase Beta-A Chain
Scs-Betaa
Database Links Reactome: R-HSA-71403
Cellular Localisation Mitochondrion
Alternative Antibody Names Anti-Succinate--Coa Ligase Adp-Forming Subunit Beta - Mitochondrial antibody
Anti-Atp-Specific Succinyl-Coa Synthetase Subunit Beta antibody
Anti-A-Scs antibody
Anti-Succinyl-Coa Synthetase Beta-A Chain antibody
Anti-Scs-Betaa antibody
Anti-SUCLA2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance