| Host: | Rabbit |
| Applications: | WB/IF/ICC/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | SUCLA2 |
| Gene ID: | 8803 |
| Uniprot ID: | SUCB1_HUMAN |
| Immunogen Region: | 1-180 |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human SUCLA2 (NP_003841.1). |
| Immunogen Sequence: | MAASMFYGRLVAVATLRNHR PRTAQRAAAQVLGSSGLFNN HGLQVQQQQQRNLSLHEYMS MELLQEAGVSVPKGYVAKSP DEAYAIAKKLGSKDVVIKAQ VLAGGRGKGTFESGLKGGVK IVFSPEEAKAVSSQMIGKKL FTKQTGEKGRICNQVLVCER KYPRREYYFAITMERSFQGP |
| Tissue Specificity | Widely expressed. Not expressed in liver and lung. |
| Function | ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit. |
| Protein Name | Succinate--Coa Ligase Adp-Forming Subunit Beta - MitochondrialAtp-Specific Succinyl-Coa Synthetase Subunit BetaA-ScsSuccinyl-Coa Synthetase Beta-A ChainScs-Betaa |
| Database Links | Reactome: R-HSA-71403 |
| Cellular Localisation | Mitochondrion |
| Alternative Antibody Names | Anti-Succinate--Coa Ligase Adp-Forming Subunit Beta - Mitochondrial antibodyAnti-Atp-Specific Succinyl-Coa Synthetase Subunit Beta antibodyAnti-A-Scs antibodyAnti-Succinyl-Coa Synthetase Beta-A Chain antibodyAnti-Scs-Betaa antibodyAnti-SUCLA2 antibody |
Information sourced from Uniprot.org
![Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/89796/358265/STJ11100862_1__27370.1713122606.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ARF5 knockout (KO) 293T cells, using [KO Validated] ARF5 Rabbit polyclonal antibody (STJ11100862) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")
