• Western blot analysis of extracts of various cell lines, using STXBP4 antibody (STJ110586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
  • Immunohistochemistry analysis of paraffin-embedded human esophagus using STXBP4 antibody (STJ110586) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded human esophagus using STXBP4 antibody (STJ110586) at dilution of 1:100 (40x lens).
  • Western blot analysis of extracts of various cell lines, using STXBP4 antibody (STJ110586) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-STXBP4 antibody (344-553) (STJ110586)

SKU:
STJ110586

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STXBP4 (344-553) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STXBP4
Gene ID: 252983
Uniprot ID: STXB4_HUMAN
Immunogen Region: 344-553
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 344-553 of human STXBP4 (NP_848604.3).
Immunogen Sequence: EETRALRSRIHLAEAAQRQA HGMEMDYEEVIRLLEAKITE LKAQLADYSDQNKESVQDLK KRIMVLDCQLRKSEMARKTF EASTEKLLHFVEAIQEVFSD NSTPLSNLSERRAVLASQTS LTPLGRNGRSIPATLALESK ELVKSVRALLDMDCLPYGWE EAYTADGIKYFINHVTQTTS WIHPVMSVLNLSRSEENEED CSRELPNQKS
Post Translational Modifications Phosphorylated on Ser-99 by PKB/AKT2 after insulin treatment. Phosphorylation on Ser-99 abolishes the interaction with STX4A.
Function Plays a role in the translocation of transport vesicles from the cytoplasm to the plasma membrane. Inhibits the translocation of SLC2A4 from intracellular vesicles to the plasma membrane by STX4A binding and preventing the interaction between STX4A and VAMP2. Stimulation with insulin disrupts the interaction with STX4A, leading to increased levels of SLC2A4 at the plasma membrane. May also play a role in the regulation of insulin release by pancreatic beta cells after stimulation by glucose.
Protein Name Syntaxin-Binding Protein 4
Syntaxin 4-Interacting Protein
Stx4-Interacting Protein
Synip
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Syntaxin-Binding Protein 4 antibody
Anti-Syntaxin 4-Interacting Protein antibody
Anti-Stx4-Interacting Protein antibody
Anti-Synip antibody
Anti-STXBP4 antibody

Information sourced from Uniprot.org

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