• Western blot analysis of extracts of HeLa cells, using STXBP1 rabbit monoclonal antibody (STJ11102476) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
  • Immunohistochemistry analysis of STXBP1 in paraffin-embedded human brain tissue using STXBP1 rabbit monoclonal antibody (STJ11102476) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of STXBP1 in paraffin-embedded human pancreas tissue using STXBP1 rabbit monoclonal antibody (STJ11102476) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of STXBP1 in paraffin-embedded mouse pancreas tissue using STXBP1 rabbit monoclonal antibody (STJ11102476) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of STXBP1 in paraffin-embedded rat pancreas tissue using STXBP1 rabbit monoclonal antibody (STJ11102476) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-STXBP1 antibody (400-500) [S6MR] (STJ11102476)

SKU:
STJ11102476

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-STXBP1 (400-500) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S6MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STXBP1
Gene ID: 6812
Uniprot ID: STXB1_HUMAN
Immunogen Region: 400-500
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 400-500 of human STXBP1 (P61764).
Immunogen Sequence: STYDKIRIILLYIFLKNGIT EENLNKLIQHAQIPPEDSEI ITNMAHLGVPIVTDSTLRRR SKPERKERISEQTYQLSRWT PIIKDIMEDTIEDKLDTKHY P
Tissue Specificity Brain and spinal cord. Highly enriched in axons.
Function Participates in the regulation of synaptic vesicle docking and fusion through interaction with GTP-binding proteins. Essential for neurotransmission and binds syntaxin, a component of the synaptic vesicle fusion machinery probably in a 1:1 ratio. Can interact with syntaxins 1, 2, and 3 but not syntaxin 4. Involved in the release of neurotransmitters from neurons through interacting with SNARE complex component STX1A and mediating the assembly of the SNARE complex at synapic membranes. May play a role in determining the specificity of intracellular fusion reactions.
Protein Name Syntaxin-Binding Protein 1
Munc18-1
N-Sec1
Protein Unc-18 Homolog 1
Unc18-1
Protein Unc-18 Homolog A
Unc-18a
P67
Database Links Reactome: R-HSA-181429 P61764-1
Reactome: R-HSA-181430 P61764-1
Reactome: R-HSA-210500 P61764-1
Reactome: R-HSA-212676 P61764-1
Reactome: R-HSA-264642 P61764-1
Reactome: R-HSA-422356
Reactome: R-HSA-6794361
Reactome: R-HSA-888590 P61764-1
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative Antibody Names Anti-Syntaxin-Binding Protein 1 antibody
Anti-Munc18-1 antibody
Anti-N-Sec1 antibody
Anti-Protein Unc-18 Homolog 1 antibody
Anti-Unc18-1 antibody
Anti-Protein Unc-18 Homolog A antibody
Anti-Unc-18a antibody
Anti-P67 antibody
Anti-STXBP1 antibody
Anti-UNC18A antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance