• Western blot analysis of extracts of various cell lines, using STX8 antibody (STJ112090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STX8 antibody (1-215) (STJ112090)

SKU:
STJ112090

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STX8 (1-215) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX8
Gene ID: 9482
Uniprot ID: STX8_HUMAN
Immunogen Region: 1-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-215 of human STX8 (NP_004844.1).
Immunogen Sequence: MAPDPWFSTYDSTCQIAQEI AEKIQQRNQYERKGEKAPKL TVTIRALLQNLKEKIALLKD LLLRAVSTHQITQLEGDRRQ NLLDDLVTRERLLLASFKNE GAEPDLIRSSLMSEEAKRGA PNPWLFEEPEETRGLGFDEI RQQQQKIIQEQDAGLDALSS IISRQKQMGQEIGNELDEQN EIIDDLANLVENTDEKLRNE TRRVNMVDRKSASCG
Tissue Specificity Highly expressed in heart. Also found in brain, kidney, liver, lung, placenta, skeletal muscle, spleen and pancreas.
Post Translational Modifications Ubiquitinated by HECTD3.
Function Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.
Protein Name Syntaxin-8
Cellular Localisation Membrane
Single-Pass Type Iv Membrane Protein
Preferentially Associated With The Early Endosome
To A Lesser Extent
Also Present In Late Endosome
The Plasma Membrane And Coated Pits
Alternative Antibody Names Anti-Syntaxin-8 antibody
Anti-STX8 antibody

Information sourced from Uniprot.org

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