• Immunofluorescence analysis of mouse brain using STX1A Rabbit monoclonal antibody (STJ11102975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of rat brain using STX1A Rabbit monoclonal antibody (STJ11102975) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using STX1A Rabbit monoclonal antibody (STJ11102975) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using STX1A antibody (STJ11102975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of Rat brain, using STX1A antibody (STJ11102975) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-STX1A antibody (1-100) [S5MR] (STJ11102975)

SKU:
STJ11102975

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-STX1A (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STX1A
Gene ID: 6804
Uniprot ID: STX1A_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human STX1A (Q16623).
Immunogen Sequence: MKDRTQELRTAKDSDDDDDV AVTVDRDRFMDEFFEQVEEI RGFIDKIAENVEEVKRKHSA ILASPNPDEKTKEELEELMS DIKKTANKVRSKLKSIEQSI
Tissue Specificity Isoform 1: Highly expressed in embryonic spinal cord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2: Expressed in heart, liver, fat, skeletal muscle, kidney and brain.
Post Translational Modifications Phosphorylated by CK2. Phosphorylation at Ser-188 by DAPK1 significantly decreases its interaction with STXBP1. Sumoylated, sumoylation is required for regulation of synaptic vesicle endocytosis.
Function Plays an essential role in hormone and neurotransmitter calcium-dependent exocytosis and endocytosis. Part of the SNARE (Soluble NSF Attachment Receptor) complex composed of SNAP25, STX1A and VAMP2 which mediates the fusion of synaptic vesicles with the presynaptic plasma membrane. STX1A and SNAP25 are localized on the plasma membrane while VAMP2 resides in synaptic vesicles. The pairing of the three SNAREs from the N-terminal SNARE motifs to the C-terminal anchors leads to the formation of the SNARE complex, which brings membranes into close proximity and results in final fusion. Participates in the calcium-dependent regulation of acrosomal exocytosis in sperm. Also plays an important role in the exocytosis of hormones such as insulin or glucagon-like peptide 1 (GLP-1).
Protein Name Syntaxin-1a
Neuron-Specific Antigen Hpc-1
Database Links Reactome: R-HSA-181429
Reactome: R-HSA-181430
Reactome: R-HSA-210500
Reactome: R-HSA-212676
Reactome: R-HSA-264642
Reactome: R-HSA-264876
Reactome: R-HSA-422356
Reactome: R-HSA-449836
Reactome: R-HSA-5250971
Reactome: R-HSA-5682910
Reactome: R-HSA-6794361
Reactome: R-HSA-888590
Reactome: R-HSA-9609523
Reactome: R-HSA-9662360
Cellular Localisation Cytoplasmic Vesicle
Secretory Vesicle
Synaptic Vesicle Membrane
Single-Pass Type Iv Membrane Protein
Synapse
Synaptosome
Cell Membrane
Colocalizes With Kcnb1 At The Cell Membrane
Isoform 2: Secreted
Alternative Antibody Names Anti-Syntaxin-1a antibody
Anti-Neuron-Specific Antigen Hpc-1 antibody
Anti-STX1A antibody
Anti-STX1 antibody

Information sourced from Uniprot.org

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