• Western blot analysis of lysates from wild type (WT) and STRADA knockout (KO) HeLa cells, using [KO Validated] STRADA Rabbit polyclonal antibody (STJ119985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-STRADA antibody (25-210) (STJ119985)

SKU:
STJ119985

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STRADA (25-210) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STRADA
Gene ID: 92335
Uniprot ID: STRAA_HUMAN
Immunogen Region: 25-210
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 25-210 of human STRADA (NP_699166.2).
Immunogen Sequence: FLPEGGCYELLTVIGKGFED LMTVNLARYKPTGEYVTVRR INLEACSNEMVTFLQGELHV SKLFNHPNIVPYRATFIADN ELWVVTSFMAYGSAKDLICT HFMDGMNELAIAYILQGVLK ALDYIHHMGYVHRSVKASHI LISVDGKVYLSGLRSNLSMI SHGQRQRVVHDFPKYSVKVL PWLSPE
Function Pseudokinase which, in complex with CAB39/MO25 (CAB39/MO25alpha or CAB39L/MO25beta), binds to and activates STK11/LKB1. Adopts a closed conformation typical of active protein kinases and binds STK11/LKB1 as a pseudosubstrate, promoting conformational change of STK11/LKB1 in an active conformation.
Protein Name Ste20-Related Kinase Adapter Protein Alpha
Strad Alpha
Ste20-Related Adapter Protein
Serologically Defined Breast Cancer Antigen Ny-Br-96
Database Links Reactome: R-HSA-380972
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Ste20-Related Kinase Adapter Protein Alpha antibody
Anti-Strad Alpha antibody
Anti-Ste20-Related Adapter Protein antibody
Anti-Serologically Defined Breast Cancer Antigen Ny-Br-96 antibody
Anti-STRADA antibody
Anti-LYK5 antibody
Anti-STRAD antibody

Information sourced from Uniprot.org

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