• Western blot analysis of various lysates, using STK38 rabbit polyclonal antibody (STJ110490) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using STK38 rabbit polyclonal antibody (STJ110490) at 1:800 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Immunofluorescence analysis of U2OS cells using STK38 antibody (STJ110490) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using STK38 antibody (STJ110490) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-STK38 antibody (418-465) (STJ110490)

SKU:
STJ110490

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STK38 (418-465) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK38
Gene ID: 11329
Uniprot ID: STK38_HUMAN
Immunogen Region: 418-465
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 418-465 of human STK38 (NP_009202.1).
Immunogen Sequence: ILKPTVATSNHPETDYKN
Tissue Specificity Ubiquitously expressed with highest levels observed in peripheral blood leukocytes.
Post Translational Modifications ISGylated. Phosphorylated by STK3/MST2 and this is enhanced by MOBKL1B.
Function Negative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its non-phosphorylated form and inhibits autophosphorylation of MAP3K2.
Protein Name Serine/Threonine-Protein Kinase 38
Ndr1 Protein Kinase
Nuclear Dbf2-Related Kinase 1
Database Links Reactome: R-HSA-9013418
Reactome: R-HSA-9013422
Cellular Localisation Nucleus
Cytoplasm
Alternative Antibody Names Anti-Serine/Threonine-Protein Kinase 38 antibody
Anti-Ndr1 Protein Kinase antibody
Anti-Nuclear Dbf2-Related Kinase 1 antibody
Anti-STK38 antibody
Anti-NDR1 antibody

Information sourced from Uniprot.org

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