• Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using STK19 Rabbit polyclonal antibody (STJ115345) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using STK19 antibody (STJ115345) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-STK19 antibody (75-364) (STJ115345)

SKU:
STJ115345

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STK19 (75-364) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STK19
Gene ID: 8859
Uniprot ID: STK19_HUMAN
Immunogen Region: 75-364
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 75-364 of human STK19 (NP_004188.1).
Immunogen Sequence: GTPGETVRHCSAPEDPIFRF SSLHSYPFPGTIKSRDMSWK RHHLIPETFGVKRRRKRGPV ESDPLRGEPGSARAAVSELM QLFPRGLFEDALPPIVLRSQ VYSLVPDRTVADRQLKELQE QGEIRIVQLGFDLDAHGIIF TEDYRTRVLKACDGRPYAGA VQKFLASVLPACGDLSFQQD QMTQTFGFRDSEITHLVNAG VLTVRDAGSWWLAVPGAGRF IKYFVKGRQAVLSMVRKAK
Tissue Specificity Monocytes, hepatocytes, epithelial cells, T- and B-lymphocytes.
Function Isoform 1: Inactive serine/threonine-protein kinase (Probable). May control NRAS activity via an associated kinase (Probable). Isoform 3: Inactive serine/threonine-protein kinase.
Protein Name Inactive Serine/Threonine-Protein Kinase 19
Protein G11
Protein Rp1
Cellular Localisation Isoform 3: Nucleus
Very Tightly Chromatin-Associated
Isoform 1: Nucleus
Cytoplasm
Alternative Antibody Names Anti-Inactive Serine/Threonine-Protein Kinase 19 antibody
Anti-Protein G11 antibody
Anti-Protein Rp1 antibody
Anti-STK19 antibody
Anti-G11 antibody
Anti-RP1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance