• Immunoprecipitation analysis of 200 Mu g extracts of HeLa cells using 1 Mu g STIP1 antibody ( STJ116041). Western blot was performed from the immunoprecipitate using STIP1 antibody ( STJ116041) at a dilution of 1:1000.
  • Immunofluorescence analysis of HeLa cells using STIP1 Rabbit polyclonal antibody (STJ116041). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of STIP1 in paraffin-embedded mouse testis using STIP1 Rabbit polyclonal antibody (STJ116041) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of STIP1 in paraffin-embedded rat testis using STIP1 Rabbit polyclonal antibody (STJ116041) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using STIP1 Rabbit polyclonal antibody (STJ116041) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Anti-STIP1 antibody (1-300) (STJ116041)

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STJ116041

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STIP1 (1-300) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STIP1
Gene ID: 10963
Uniprot ID: STIP1_HUMAN
Immunogen Region: 1-300
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human STIP1 (NP_006810.1).
Immunogen Sequence: MEQVNELKEKGNKALSVGNI DDALQCYSEAIKLDPHNHVL YSNRSAAYAKKGDYQKAYED GCKTVDLKPDWGKGYSRKAA ALEFLNRFEEAKRTYEEGLK HEANNPQLKEGLQNMEARLA ERKFMNPFNMPNLYQKLESD PRTRTLLSDPTYRELIEQLR NKPSDLGTKLQDPRIMTTLS VLLGVDLGSMDEEEEIATPP PPPPPKKETKPEPMEEDLPE NKKQALKEKELGNDAYKKK
Function Acts as a co-chaperone for HSP90AA1. Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90.
Protein Name Stress-Induced-Phosphoprotein 1
Sti1
Hsc70/Hsp90-Organizing Protein
Hop
Renal Carcinoma Antigen Ny-Ren-11
Transformation-Sensitive Protein Ief Ssp 3521
Database Links Reactome: R-HSA-3371497
Reactome: R-HSA-9696273
Cellular Localisation Cytoplasm
Nucleus
Dynein Axonemal Particle
Alternative Antibody Names Anti-Stress-Induced-Phosphoprotein 1 antibody
Anti-Sti1 antibody
Anti-Hsc70/Hsp90-Organizing Protein antibody
Anti-Hop antibody
Anti-Renal Carcinoma Antigen Ny-Ren-11 antibody
Anti-Transformation-Sensitive Protein Ief Ssp 3521 antibody
Anti-STIP1 antibody

Information sourced from Uniprot.org

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