| Host: |
Goat |
| Applications: |
IHC/WB |
| Reactivity: |
Human/Rat/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-STIM1 (ECD) is suitable for use in Immunohistochemistry and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Lyophilised |
| Purification: |
IgG purified |
| Dilution Range: |
IHC, WB. A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not tested in other applications. |
| Storage Instruction: |
Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
| Gene Symbol: |
STIM1 |
| Gene ID: |
6786 |
| Uniprot ID: |
STIM1_HUMAN |
| Immunogen Region: |
ECD |
| Specificity: |
Specific for STIM1. |
| Immunogen: |
A synthetic peptide from the extracellular domain of human Stromal interaction molecule 1 (STIM1) conjugated to blue carrier protein was used as the antigen. |
| Post Translational Modifications | Glycosylation is required for cell surface expression. Phosphorylated predominantly on Ser residues. |
| Function | Acts as a Ca(2+) sensor that gates two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates CRAC channel pore-forming subunits ORA1, ORA2 and ORAI3 to generate sustained and oscillatory Ca(2+) entry. Involved in enamel formation. |
| Protein Name | Stromal Interaction Molecule 1 |
| Database Links | Reactome: R-HSA-139853Reactome: R-HSA-5578775Reactome: R-HSA-983695 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneCytoplasmCytoskeletonSarcoplasmic ReticulumTranslocates From The Endoplasmic Reticulum To The Cell Membrane In Response To A Depletion Of Intracellular Calcium And Is Detected At Punctae Corresponding To Junctions Between The Endoplasmic Reticulum And The Cell MembraneAssociated With The Microtubule Network At The Growing Distal Tip Of MicrotubulesColocalizes With Orai1 At The Cell MembraneColocalizes Preferentially With Casq1 At Endoplasmic Reticulum In Response To A Depletion Of Intracellular Calcium |
| Alternative Antibody Names | Anti-Stromal Interaction Molecule 1 antibodyAnti-STIM1 antibodyAnti-GOK antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89707/358189/STJ11100770_1__47615.1713122504.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STIM1 knockout (KO) HeLa cells, using [KO Validated] STIM1 Rabbit polyclonal antibody (STJ11100770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")