• Western blot analysis of extracts of various cell lines, using STAT6 antibody (STJ25726) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of HeLa cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using STAT6 rabbit polyclonal antibody (STJ25726) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-STAT6 antibody (501-600) (STJ25726)

SKU:
STJ25726

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STAT6 (501-600) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT6
Gene ID: 6778
Uniprot ID: STAT6_HUMAN
Immunogen Region: 501-600
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 501-600 of human STAT6 (NP_003144.3).
Immunogen Sequence: AACTCCTGCTAATGGGACTG GGCCAAGTGAGGCCCTGGCC ATGCTACTGCAGGAGACCAC TGGAGAGCTAGAGGCAGCCA AAGCCCTAGTGCTGAAGAGG
Post Translational Modifications Tyrosine phosphorylated on Tyr-641 following stimulation by IL4/interleukin-4. Tyrosine phosphorylated following stimulation by IL3/interleukin-3. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates the IL4/interleukin-4 mediated signaling. Mono-ADP-ribosylated by PARP14.
Function Carries out a dual function: signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling.
Protein Name Signal Transducer And Activator Of Transcription 6
Il-4 Stat
Database Links Reactome: R-HSA-186763
Reactome: R-HSA-3249367
Reactome: R-HSA-6785807
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus In Response To Phosphorylation
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 6 antibody
Anti-Il-4 Stat antibody
Anti-STAT6 antibody

Information sourced from Uniprot.org

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