![Immunohistochemistry analysis of STAT5B in paraffin-embedded rat intestine using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368368/STJ11104439_5__59394.1713133861.jpg?c=1 "Immunohistochemistry analysis of STAT5B in paraffin-embedded rat intestine using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of STAT5B in paraffin-embedded mouse kidney using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368369/STJ11104439_6__99236.1713133862.jpg?c=1 "Immunohistochemistry analysis of STAT5B in paraffin-embedded mouse kidney using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of STAT5B in paraffin-embedded mouse intestin using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368370/STJ11104439_7__50221.1713133862.jpg?c=1 "Immunohistochemistry analysis of STAT5B in paraffin-embedded mouse intestin using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of STAT5B in paraffin-embedded human tonsil using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368371/STJ11104439_8__15932.1713133863.jpg?c=1 "Immunohistochemistry analysis of STAT5B in paraffin-embedded human tonsil using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of STAT5B in paraffin-embedded human colon carcinoma using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368372/STJ11104439_9__87641.1713133864.jpg?c=1 "Immunohistochemistry analysis of STAT5B in paraffin-embedded human colon carcinoma using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368373/STJ11104439_10__92083.1713133865.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and STAT5B knockout (KO) HeLa cells, using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Western blot analysis of various lysates using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/177083/368374/STJ11104439_11__09055.1713133866.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] STAT5B Rabbit polyclonal antibody (STJ11104439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
| Short Description : | Rabbit polyclonal Signal Transducer And Activator Of Transcription 5b (586-787) antibody for use in WB, IHC-P, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications: | WB/IHC-P/IF/ICC/IP/ELISA |
| Host: | Rabbit |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | Lot specific |
| Purification: | Affinity purification |
| Dilution Range: | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 400 Mu g-600 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | STAT5B |
| Gene ID: | 6777 |
| Uniprot ID: | STA5B_HUMAN |
| Immunogen Region: | 586-787 |
| Immunogen Sequence: | KPHWNDGAILGFVNKQQAHD LLINKPDGTFLLRFSDSEIG GITIAWKFDSQERMFWNLMP FTTRDFSIRSLADRLGDLNY LIYVFPDRPKDEVYSKYYTP VPCESATAKAVDGYVKPQIK QVVPEFVNASADAGGGSATY MDQAPSPAVCPQAHYNMYPQ NPDSVLDTDGDFDLEDTMDV ARRVEELLGRPMDSQWIPHA QS |
| Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 586-787 of human STAT5B (NP_036580.2). |
| Post Translational Modifications | Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3.wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. |
| Function | Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation. |
| Protein Name | Signal Transducer And Activator Of Transcription 5b |
| Database Links | Reactome: R-HSA-1170546Reactome: R-HSA-1266695Reactome: R-HSA-1433557Reactome: R-HSA-1839117Reactome: R-HSA-186763Reactome: R-HSA-2586552Reactome: R-HSA-512988Reactome: R-HSA-8854691Reactome: R-HSA-8983432Reactome: R-HSA-8985947Reactome: R-HSA-9020558Reactome: R-HSA-9020958Reactome: R-HSA-9027283Reactome: R-HSA-9645135Reactome: R-HSA-9670439Reactome: R-HSA-9674555Reactome: R-HSA-9702518Reactome: R-HSA-9703465Reactome: R-HSA-9705462Reactome: R-HSA-982772 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus In Response To Phosphorylation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 5b antibodyAnti-STAT5B antibody |
Information sourced from Uniprot.org
