Anti-STAT4 antibody (649-748) [S4MR] (STJ11102094)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit monoclonal STAT4 (649-748) antibody for use in WB, IF, ICC, IP and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/IP/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | S4MR |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements |
| Formulation | PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | STAT4 |
| Gene ID | 6775 |
| Uniprot ID | STAT4_HUMAN |
| Immunogen Region | 649-748 |
| Immunogen Sequence | MAENIPENPLKYLYPDIPKD KAFGKHYSSQPCEVSRPTER GDKGYVPSVFIPISTIRSDS TEPHSPSDLLPMSPSVYAVL RENLSPTTIETAMKSPYSAE |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 649-748 of human STAT4 (NP_003142.1). |
Additional Info
| Post Translational Modifications | Acetylation at Lys-667 is required for JAK2-mediated phosphorylation and activation of STAT4. Tyrosine phosphorylated upon IL12 and IFN-alpha activation, but not by IFN-gamma in T-lymphocytes and NK cells. Serine phosphorylation is required for maximal transcriptional activity but not for DNA binding. Phosphorylation by MAP2K6 at Ser-721 is required for full transcriptional activity induced by IL12. However this serine phosphorylation is not required for cell proliferation although critical for IFN-gamma production. |
| Function | Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Also participates in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35. Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter. In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation. |
| Protein Name | Signal Transducer And Activator Of Transcription 4 |
| Database Links | Reactome: R-HSA-8854691Reactome: R-HSA-8950505Reactome: R-HSA-8984722Reactome: R-HSA-9020591Reactome: R-HSA-9020933Reactome: R-HSA-9020958 |
| Cellular Localisation | CytoplasmNucleusTranslocated Into The Nucleus In Response To Phosphorylation |
| Alternative Antibody Names | Anti-Signal Transducer And Activator Of Transcription 4 antibodyAnti-STAT4 antibody |
Information sourced from Uniprot.org