• Immunohistochemistry analysis of STAT4 in paraffin-embedded Rat testis using STAT4 Rabbit polyclonal antibody (STJ25719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of STAT4 in paraffin-embedded Mouse spleen using STAT4 Rabbit polyclonal antibody (STJ25719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of STAT4 in paraffin-embedded Human colon using STAT4 Rabbit polyclonal antibody (STJ25719) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using STAT4 Rabbit polyclonal antibody (STJ25719) at 1:3000 dilution. _Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. _Lysates/proteins: 25 Mu g per lane. _Blocking buffer: 3% nonfat dry milk in TBST. _Detection: ECL Enhanced Kit. _Exposure time: 90s.

Anti-STAT4 antibody (569-748) (STJ25719)

SKU:
STJ25719

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STAT4 (569-748) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAT4
Gene ID: 6775
Uniprot ID: STAT4_HUMAN
Immunogen Region: 569-748
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 569-748 of human STAT4 (NP_003142.1).
Immunogen Sequence: WIDGYVMGFVSKEKERLLLK DKMPGTFLLRFSESHLGGIT FTWVDHSESGEVRFHSVEPY NKGRLSALPFADILRDYKVI MAENIPENPLKYLYPDIPKD KAFGKHYSSQPCEVSRPTER GDKGYVPSVFIPISTIRSDS TEPHSPSDLLPMSPSVYAVL RENLSPTTIETAMKSPYSAE
Post Translational Modifications Acetylation at Lys-667 is required for JAK2-mediated phosphorylation and activation of STAT4. Tyrosine phosphorylated upon IL12 and IFN-alpha activation, but not by IFN-gamma in T-lymphocytes and NK cells. Serine phosphorylation is required for maximal transcriptional activity but not for DNA binding. Phosphorylation by MAP2K6 at Ser-721 is required for full transcriptional activity induced by IL12. However this serine phosphorylation is not required for cell proliferation although critical for IFN-gamma production.
Function Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35. Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter. In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation.
Protein Name Signal Transducer And Activator Of Transcription 4
Database Links Reactome: R-HSA-8854691
Reactome: R-HSA-8950505
Reactome: R-HSA-8984722
Reactome: R-HSA-9020591
Reactome: R-HSA-9020933
Reactome: R-HSA-9020958
Cellular Localisation Cytoplasm
Nucleus
Translocated Into The Nucleus In Response To Phosphorylation
Alternative Antibody Names Anti-Signal Transducer And Activator Of Transcription 4 antibody
Anti-STAT4 antibody

Information sourced from Uniprot.org

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