• Western blot analysis of lysates from HT-29 cells, using STAP2 Rabbit polyclonal antibody (STJ118571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 240s.
  • Western blot analysis of lysates from Mouse heart, using STAP2 Rabbit polyclonal antibody (STJ118571) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-STAP2 antibody (130-220) (STJ118571)

SKU:
STJ118571

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-STAP2 (130-220) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: STAP2
Gene ID: 55620
Uniprot ID: STAP2_HUMAN
Immunogen Region: 130-220
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 130-220 of human STAP2 (NP_001013863).
Immunogen Sequence: GHLYMMSEVLAKEEARRALE TPSCFLKVSRLEAQLLLERY PECGNLLLRPSGDGADGVSV TTRQMHNGTHVVRHYKVKRE GPKYVIDVEQP
Tissue Specificity Widely expressed.
Post Translational Modifications Phosphorylated on tyrosine. Tyr-250 may be important for interaction with kinases. Phosphorylated by PTK6 at Tyr-250 modulates PTK6-mediated STAT3 activation. Tyr-22 and Tyr-322 appears to be phosphorylated by SRC.
Function Substrate of protein kinase PTK6. May play a regulatory role in the acute-phase response in systemic inflammation and may modulate STAT3 activity.
Protein Name Signal-Transducing Adaptor Protein 2
Stap-2
Breast Tumor Kinase Substrate
Brk Substrate
Database Links Reactome: R-HSA-8849474
Reactome: R-HSA-9707564
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Signal-Transducing Adaptor Protein 2 antibody
Anti-Stap-2 antibody
Anti-Breast Tumor Kinase Substrate antibody
Anti-Brk Substrate antibody
Anti-STAP2 antibody
Anti-BKS antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance