| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SSRP1 (1-300) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SSRP1 |
| Gene ID: |
6749 |
| Uniprot ID: |
SSRP1_HUMAN |
| Immunogen Region: |
1-300 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human SSRP1 (NP_003137.1). |
| Immunogen Sequence: |
MAETLEFNDVYQEVKGSMND GRLRLSRQGIIFKNSKTGKV DNIQAGELTEGIWRRVALGH GLKLLTKNGHVYKYDGFRES EFEKLSDFFKTHYRLELMEK DLCVKGWNWGTVKFGGQLLS FDIGDQPVFEIPLSNVSQCT TGKNEVTLEFHQNDDAEVSL MEVRFYVPPTQEDGVDPVEA FAQNVLSKADVIQATGDAIC IFRELQCLTPRGRYDIRIYP TFLHLHGKTFDYKIPYTTV |
| Post Translational Modifications | Phosphorylated by CK2 following UV but not gamma irradiation. Phosphorylation inhibits its DNA-binding activity. Ubiquitinated. Polyubiquitinated following caspase cleavage resulting in degradation of the N-terminal ubiquitinated part of the cleaved protein. Sumoylated. |
| Function | Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA and at low levels to DNA modified by the antitumor agent cisplatin. May potentiate cisplatin-induced cell death by blocking replication and repair of modified DNA. Also acts as a transcriptional coactivator for p63/TP63. |
| Protein Name | Fact Complex Subunit Ssrp1Chromatin-Specific Transcription Elongation Factor 80 Kda SubunitFacilitates Chromatin Transcription Complex 80 Kda SubunitFact 80 Kda SubunitFactp80Facilitates Chromatin Transcription Complex Subunit Ssrp1Recombination Signal Sequence Recognition Protein 1Structure-Specific Recognition Protein 1Hssrp1T160 |
| Database Links | Reactome: R-HSA-112382Reactome: R-HSA-167152Reactome: R-HSA-167200Reactome: R-HSA-167238Reactome: R-HSA-167243Reactome: R-HSA-167246Reactome: R-HSA-167287Reactome: R-HSA-167290Reactome: R-HSA-674695Reactome: R-HSA-6796648Reactome: R-HSA-6804756Reactome: R-HSA-75955 |
| Cellular Localisation | NucleusNucleolusChromosomeColocalizes With Rna Polymerase Ii On ChromatinRecruited To Actively Transcribed Loci |
| Alternative Antibody Names | Anti-Fact Complex Subunit Ssrp1 antibodyAnti-Chromatin-Specific Transcription Elongation Factor 80 Kda Subunit antibodyAnti-Facilitates Chromatin Transcription Complex 80 Kda Subunit antibodyAnti-Fact 80 Kda Subunit antibodyAnti-Factp80 antibodyAnti-Facilitates Chromatin Transcription Complex Subunit Ssrp1 antibodyAnti-Recombination Signal Sequence Recognition Protein 1 antibodyAnti-Structure-Specific Recognition Protein 1 antibodyAnti-Hssrp1 antibodyAnti-T160 antibodyAnti-SSRP1 antibodyAnti-FACT80 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89098/357557/STJ11100026_1__69013.1713121681.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and BUB1 knockout (KO) HeLa cells, using [KO Validated] BUB1 Rabbit polyclonal antibody (STJ11100026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.")