• Immunohistochemistry analysis of paraffin-embedded mouse kidney using SSNA1 Rabbit polyclonal antibody (STJ115339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human breast cancer using SSNA1 Rabbit polyclonal antibody (STJ115339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat lung using SSNA1 Rabbit polyclonal antibody (STJ115339) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of mouse spleen, using SSNA1 antibody (STJ115339) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-SSNA1 antibody (1-119) (STJ115339)

SKU:
STJ115339

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SSNA1 (1-119) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SSNA1
Gene ID: 8636
Uniprot ID: SSNA1_HUMAN
Immunogen Region: 1-119
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human SSNA1 (NP_003722.2).
Immunogen Sequence: MTQQGAALQNYNNELVKCIE ELCQKREELCRQIQEEEDEK QRLQNEVRQLTEKLARVNEN LARKIASRNEFDRTIAETEA AYLKILESSQTLLSVLKREA GNLTKATAPDQKSSGGRDS
Tissue Specificity Widely expressed.
Function Microtubule-binding protein which stabilizes dynamic microtubules by slowing growth and shrinkage at both plus and minus ends and serves as a sensor of microtubule damage, protecting microtubules from the microtubule-severing enzyme SPAST. Induces microtubule branching which is mediated by the formation of long SSNA1 fibrils which guide microtubule protofilaments to split apart from the mother microtubule and form daughter microtubules. Plays a role in axon outgrowth and branching. Required for cell division.
Protein Name Microtubule Nucleation Factor Ssna1
Nuclear Autoantigen Of 14 Kda
Sjoegren Syndrome Nuclear Autoantigen 1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Nucleus
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Midbody
Flagellum Basal Body
Flagellum Axoneme
Cell Projection
Axon
In Sperm
Strongly Expressed In The Basal Body Region With Weaker Expression In The Axoneme
Localizes To Axon Branching Points In Neurons
Alternative Antibody Names Anti-Microtubule Nucleation Factor Ssna1 antibody
Anti-Nuclear Autoantigen Of 14 Kda antibody
Anti-Sjoegren Syndrome Nuclear Autoantigen 1 antibody
Anti-SSNA1 antibody
Anti-NA14 antibody

Information sourced from Uniprot.org

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