• Immunoprecipitation analysis of 200 Mu g extracts of MCF7 cells using 1 Mu g SSBP1 antibody (STJ29067). Western blot was performed from the immunoprecipitate using SSBP1 antibody (STJ29067) at a dilution of 1:1000.
  • Confocal immunofluorescence analysis of U2OS cells using SSBP1 Rabbit polyclonal antibody (STJ29067) at dilution of 1:100. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using SSBP1 Rabbit polyclonal antibody (STJ29067). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse liver, using SSBP1 Rabbit polyclonal antibody (STJ29067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
  • Western blot analysis of various lysates using SSBP1 Rabbit polyclonal antibody (STJ29067) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-SSBP1 antibody (1-148) (STJ29067)

SKU:
STJ29067

$228.83 - $606.83
Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SSBP1 (1-148) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
IP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SSBP1
Gene ID: 6742
Uniprot ID: SSBP_HUMAN
Immunogen Region: 1-148
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1).
Immunogen Sequence: MFRRPVLQVLRQFVRHESET TTSLVLERSLNRVHLLGRVG QDPVLRQVEGKNPVTIFSLA TNEMWRSGDSEVYQLGDVSQ KTTWHRISVFRPGLRDVAYQ YVKKGSRIYLEGKIDYGEYM DKNNVRRQATTIIADNIIFL SDQTKEKE
Tissue Specificity Expressed in retinal ganglion cells, photoreceptors, pigmented epithelium and fibroblasts (at protein level).
Function Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.
Protein Name Single-Stranded Dna-Binding Protein - Mitochondrial
Mt-Ssb
Mtssb
Pwp1-Interacting Protein 17
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion
Mitochondrion Matrix
Mitochondrion Nucleoid
Alternative Antibody Names Anti-Single-Stranded Dna-Binding Protein - Mitochondrial antibody
Anti-Mt-Ssb antibody
Anti-Mtssb antibody
Anti-Pwp1-Interacting Protein 17 antibody
Anti-SSBP1 antibody
Anti-SSBP antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance