Anti-GRP antibody (1-148) (STJ28463)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal GRP (1-148) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | GRP |
| Gene ID | 2922 |
| Uniprot ID | GRP_HUMAN |
| Immunogen Region | 1-148 |
| Immunogen Sequence | MRGRELPLVLLALVLCLAPR GRAVPLPAGGGTVLTKMYPR GNHWAVGHLMGKKSTGESSS VSERGSLKQQLREYIRWEEA ARNLLGLIEAKENRNHQPPQ PKALGNQQPSWDSEDSSNFK DVGSKGKVGRLSAPGSQREG RNPQLNQQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human Gastrin Releasing Peptide (GRP) (NP_002082.2). |
Additional Info
| Function | Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear. In another study, shown to act on vasoactive intestinal peptide (VIP)-expressing cells in the auditory cortex, most likely via extrasynaptic diffusion from local and long-range sources, to mediate disinhibition of glutamatergic cells via VIP cell-specific GRPR signaling which leads to enhanced auditory fear memories. Contributes to the regulation of food intake. Inhibits voltage-gated sodium channels but enhances voltage-gated potassium channels in hippocampal neurons. Induces sighing by acting directly on the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity. Neuromedin-C: Induces an itch response through activation of receptors present on mast cells, triggering mast cell degranulation. |
| Protein Name | Gastrin-Releasing PeptideGrp Cleaved Into - Neuromedin-CGrp-10Grp18-27 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-381771Reactome: R-HSA-416476 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory Vesicle LumenCell ProjectionNeuron ProjectionIn Neurons Of The Retrotrapezoid Nucleus/Parafacial Respiratory GroupExpressed On Neuron Projections Which Project Into The Pre-Botzinger Complex |
| Alternative Antibody Names | Anti-Gastrin-Releasing Peptide antibodyAnti-Grp Cleaved Into - Neuromedin-C antibodyAnti-Grp-10 antibodyAnti-Grp18-27 antibodyAnti-GRP antibody |
Information sourced from Uniprot.org