• HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with STJ70342 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ70342 (1mg/ml) in Lane B. Detected by chemiluminescence.
  • STJ70342 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing strong nuclear and some cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ70342 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-SSA1/Ro52 antibody (C-Term) (STJ70342)

SKU:
STJ70342-100

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Host: Goat
Applications: Pep-ELISA/WB-Trf/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-SSA1/Ro52 (C-Term) is suitable for use in ELISA, Western Blot, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:16000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: TRIM21
Gene ID: 6737
Uniprot ID: RO52_HUMAN
Immunogen Region: C-Term
Accession Number: NP_003132.2
Immunogen Sequence: CPLNIGSQGSTDY
Post Translational Modifications Autoubiquitinated.does not lead to its proteasomal degradation. Deubiquitinated by USP4.leading to its stabilization.
Function E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by catalyzing polyubiquitin-mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma. Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy. Regulates also autophagy through FIP200/RB1CC1 ubiquitination and subsequent decreased protein stability. Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI. During viral infection, promotes cell pyroptosis by mediating 'Lys-6'-linked ubiquitination of ISG12a/IFI27, facilitating its translocation into the mitochondria and subsequent CASP3 activation. When up-regulated through the IFN/JAK/STAT signaling pathway, promotes 'Lys-27'-linked ubiquitination of MAVS, leading to the recruitment of TBK1 and up-regulation of innate immunity. Mediates 'Lys-63'-linked polyubiquitination of G3BP1 in response to heat shock, leading to stress granule disassembly.
Protein Name E3 Ubiquitin-Protein Ligase Trim21
52 Kda Ro Protein
52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A
Ring Finger Protein 81
Ro(Ss-A
Sjoegren Syndrome Type A Antigen
Ss-A
Tripartite Motif-Containing Protein 21
Database Links Reactome: R-HSA-1834941
Reactome: R-HSA-3134975
Reactome: R-HSA-877300
Reactome: R-HSA-9755511
Reactome: R-HSA-983168
Cellular Localisation Cytoplasm
Cytoplasmic Vesicle
Autophagosome
Nucleus
P-Body
Stress Granule
Enters The Nucleus Upon Exposure To Nitric Oxide
Localizes To Small Dot- Or Rod-Like Structures In The Cytoplasm
Called Processing Bodies (P-Bodies) That Are Located Underneath The Plasma Membrane And Also Diffusely In The Cytoplasm
They Are Located Along The Microtubules And Are Highly Motile In Cells
Colocalizes With Dcp2 In P-Bodies
Localizes To Stress Granules In Response To Oxidative Stress
Alternative Antibody Names Anti-E3 Ubiquitin-Protein Ligase Trim21 antibody
Anti-52 Kda Ro Protein antibody
Anti-52 Kda Ribonucleoprotein Autoantigen Ro/Ss-A antibody
Anti-Ring Finger Protein 81 antibody
Anti-Ro(Ss-A antibody
Anti-Sjoegren Syndrome Type A Antigen antibody
Anti-Ss-A antibody
Anti-Tripartite Motif-Containing Protein 21 antibody
Anti-TRIM21 antibody
Anti-RNF81 antibody
Anti-RO52 antibody
Anti-SSA1 antibody

Information sourced from Uniprot.org

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