Anti-SRY antibody (51-100 aa) (STJ95789)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human SRY at the amino acid range 51-100
General Information
| Short Description | Rabbit polyclonal anti-Sex-determining region Y protein (51-100 aa) for use in WB, IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SRY |
| Gene ID | 6736 |
| Uniprot ID | SRY_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human SRY at the amino acid range 51-100 |
| Immunogen Region | 51-100 aa |
| Specificity | SRY Polyclonal Antibody detects endogenous levels of SRY protein. |
Additional Info
| Function | Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Involved in different aspects of gene regulation including promoter activation or repression. Binds to the DNA consensus sequence 5'-ATAACAAAT-3'. SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending. Also involved in pre-mRNA splicing. In male adult brain involved in the maintenance of motor functions of dopaminergic neurons. |
| Protein Name | Sex-Determining Region Y ProteinTestis-Determining Factor |
| Database Links | Reactome: R-HSA-3769402Reactome: R-HSA-9690406 |
| Cellular Localisation | Nucleus SpeckleCytoplasmNucleusAcetylation Contributes To Its Nuclear Localization And Deacetylation By Hdac3 Induces A Cytoplasmic DelocalizationColocalizes With Sox6 In SpecklesColocalizes With Caml In The NucleusColocalizes In The Nucleus With Znf208 Isoform Krab-O And Tyrosine Hydroxylase (Th)Nuclear Import Is Facilitated By Xpo4A Protein That Usually Acts As A Nuclear Export Signal Receptor |
| Alternative Antibody Names | Anti-Sex-Determining Region Y Protein antibodyAnti-Testis-Determining Factor antibodyAnti-SRY antibodyAnti-TDF antibody |
Information sourced from Uniprot.org